Construct: ORF TRCN0000472014
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002806.1_s317c1
- Derived from:
- ccsbBroadEn_01296
- DNA Barcode:
- CAAATCTATAATCTGGGATGACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRM2 (5620)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472014
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5620 | PRM2 | protamine 2 | NM_002762.4 | 100% | 100% | |
| 2 | human | 5620 | PRM2 | protamine 2 | NM_001286359.2 | 85.9% | 82.8% | (many diffs) |
| 3 | human | 5620 | PRM2 | protamine 2 | NM_001286358.2 | 79.5% | 71.3% | 269_270insAGGCTGC;300_369del |
| 4 | human | 5620 | PRM2 | protamine 2 | NM_001286357.2 | 75.1% | 74.5% | 230A>G;231_232ins75 |
| 5 | human | 5620 | PRM2 | protamine 2 | NM_001286356.2 | 72.8% | 65.7% | 270_313del;351_420del |
| 6 | human | 5620 | PRM2 | protamine 2 | NR_104428.2 | 51.4% | 1_110del;417_595del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 135
- ORF end:
- 441
- ORF length:
- 306
- Sequence:
-
1 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGCTAGCAT CGATTGATCA ACAAGTTTGT 121 ACAAAAAAGT TGGCATGGTC CGATACCGCG TGAGGAGCCT GAGCGAACGC TCGCACGAGG 181 TGTACAGGCA GCAGTTGCAT GGGCAAGAGC AAGGACACCA CGGCCAAGAG GAGCAAGGGC 241 TGAGCCCGGA GCACGTCGAG GTCTACGAGA GGACCCATGG CCAGTCTCAC TATAGGCGCA 301 GACACTGCTC TCGAAGGAGG CTGCACCGGA TCCACAGGCG GCAGCATCGC TCCTGCAGAA 361 GGCGCAAAAG ACGCTCCTGC AGGCACCGGA GGAGGCATCG CAGAGGCTGC AGAACCAGGA 421 AGAGAACATG CAGAAGGCAC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAAA TCTATAATCT 601 GGGATGACCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt