Construct: ORF TRCN0000472031
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005585.1_s317c1
- Derived from:
- ccsbBroadEn_13365
- DNA Barcode:
- ACTCAAGCCTCCTCACCGCAAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGO3 (192669)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472031
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 192669 | AGO3 | argonaute RISC catalytic co... | NM_024852.4 | 26.3% | 25.5% | (many diffs) |
| 2 | human | 192669 | AGO3 | argonaute RISC catalytic co... | XM_005270575.4 | 26.3% | 25.5% | (many diffs) |
| 3 | human | 192669 | AGO3 | argonaute RISC catalytic co... | XM_017000523.2 | 26.3% | 25.5% | (many diffs) |
| 4 | human | 192669 | AGO3 | argonaute RISC catalytic co... | XM_011540879.3 | 24.6% | 23.8% | (many diffs) |
| 5 | mouse | 214150 | Ago3 | argonaute RISC catalytic su... | XM_006502917.3 | 25.5% | 25.6% | (many diffs) |
| 6 | mouse | 214150 | Ago3 | argonaute RISC catalytic su... | XM_011240478.2 | 25% | 25.2% | (many diffs) |
| 7 | mouse | 214150 | Ago3 | argonaute RISC catalytic su... | NM_153402.2 | 24.4% | 24.6% | (many diffs) |
| 8 | mouse | 214150 | Ago3 | argonaute RISC catalytic su... | XR_001784115.1 | 19.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 753
- ORF length:
- 687
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aatcggctcc gcaggacccg ctggggccca gcccctactc atggtgccca 121 gaagacctgg ctatggcacc atgggcaaac ccattaaact gctggctaac tgttttcaag 181 ttgaaatccc aaagattgat gtctacctct atgaggtaga tattaaacca gacaagtgtc 241 cTAGGAGAGT GAACAGGGAG GTGGTTGACT CAATGGTTCA GCATTTTAAA GTAACTATAT 301 TTGGAGACCG TAGACCAGTT TATAATGGAA AAAGAAGTCT TTACACCGCC AATCCACTTC 361 CTGTGGCAAC TACAGGGGTA GATTTAGACG TTACTTTACC TGGGGAAGGT GGAAAAGATC 421 GACCTTTCAA GGTGTCAATC AAATTTGTCT CTCGGGTGAG TTGGCACCTA CTGCATGAAG 481 TACTGACAGG ACGGACCTTG CCTGAGCCAC TGGAATTAGA CAAGCCAATC AGCACTAACC 541 CTGTCCATGC CGTTGATGTG GTGCTACGAC ATCTGCCCTC CATGAAATAC ACACCTGTGG 601 GGCGTTCATT TTTCTCCGCT CCAGAAGGAT ATGACCACCC TCTGGGAGGG GGCAGGGAAG 661 TGTGGTTTGG ATTCCATCAG TCTGTTCGGC CTGCCATGTG GAAAATGATG CTTAATATCG 721 ATGAAAGAGA CCTCTGGCAG CAGTGTGGAG AATACCCAAC TTTCTTGTAC AAAGTGGTTG 781 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 841 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 901 TCAAGCCTCC TCACCGCAAG CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 961 ttgtgaaaga tt