Transcript: Human XM_005270575.4

PREDICTED: Homo sapiens argonaute RISC catalytic component 3 (AGO3), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AGO3 (192669)
Length:
8622
CDS:
5315..7897

Additional Resources:

NCBI RefSeq record:
XM_005270575.4
NBCI Gene record:
AGO3 (192669)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005270575.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000421882 AGACATCACACTCGATTATTT pLKO_005 7445 CDS 100% 15.000 21.000 N AGO3 n/a
2 TRCN0000416716 AGAGAACAGTAGCGCAGTATT pLKO_005 6231 CDS 100% 13.200 18.480 N AGO3 n/a
3 TRCN0000425075 CGCTTAAATAGTCCAAGTATA pLKO_005 7891 CDS 100% 13.200 18.480 N AGO3 n/a
4 TRCN0000007869 CGGGAACTTCTTATTCAATTT pLKO.1 7256 CDS 100% 13.200 18.480 N AGO3 n/a
5 TRCN0000415112 GTAGATTTAGACGTTACTTTA pLKO_005 5627 CDS 100% 13.200 18.480 N AGO3 n/a
6 TRCN0000007870 CCAAGATACCTTACGCACAAT pLKO.1 7864 CDS 100% 4.950 6.930 N AGO3 n/a
7 TRCN0000009637 CCTGCCACTAGAAGTCTGTAA pLKO.1 6331 CDS 100% 4.950 6.930 N Ago3 n/a
8 TRCN0000011204 CGGGATGAAATGGCTCATGTA pLKO.1 6521 CDS 100% 4.950 6.930 N AGO3 n/a
9 TRCN0000429285 GACTGATTCTCATCGGGTAAA pLKO_005 6067 CDS 100% 10.800 8.640 N Ago3 n/a
10 TRCN0000426527 GATGCTTAATATCGATGTTTC pLKO_005 5956 CDS 100% 10.800 8.640 N AGO3 n/a
11 TRCN0000416338 GCAGTTTAGGCAGGTATTATA pLKO_005 7339 CDS 100% 15.000 10.500 N AGO3 n/a
12 TRCN0000011808 CAAGTTGAAATCCCAAAGATT pLKO.1 5426 CDS 100% 5.625 3.938 N Ago3 n/a
13 TRCN0000007868 GCGGTCTCCTATAGGAAGTAT pLKO.1 8120 3UTR 100% 5.625 3.938 N AGO3 n/a
14 TRCN0000007871 CCTACAGCTTATTATCGTCAT pLKO.1 6859 CDS 100% 4.050 2.835 N AGO3 n/a
15 TRCN0000204533 CCTCCCAAAGTGCTGGAATTA pLKO.1 2856 5UTR 100% 13.200 6.600 Y LRRC74B n/a
16 TRCN0000009636 CCTGGAATAACCTACATTGTT pLKO.1 7415 CDS 100% 5.625 3.938 N Ago3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005270575.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11852 pDONR223 100% 32.7% 39.7% None (many diffs) n/a
2 ccsbBroad304_11852 pLX_304 0% 32.7% 39.7% V5 (many diffs) n/a
3 ccsbBroadEn_13365 pDONR223 100% 26.3% 25.5% None (many diffs) n/a
4 ccsbBroad304_13365 pLX_304 97.1% 26.3% 25.5% V5 (many diffs) n/a
5 TRCN0000472031 ACTCAAGCCTCCTCACCGCAAGCC pLX_317 73.8% 26.3% 25.5% V5 (many diffs) n/a
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