Construct: ORF TRCN0000472034
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014911.1_s317c1
- Derived from:
- ccsbBroadEn_00906
- DNA Barcode:
- TTAACGAAAAACTACGGATGGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KEL (3792)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472034
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3792 | KEL | Kell metallo-endopeptidase ... | NM_000420.3 | 100% | 100% | |
| 2 | human | 3792 | KEL | Kell metallo-endopeptidase ... | XM_005249993.2 | 98.3% | 98.2% | 1_26del;29_38delTCAGGGGACA |
| 3 | human | 3792 | KEL | Kell metallo-endopeptidase ... | XM_005249994.3 | 57.7% | 57.7% | 0_1ins927 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2262
- ORF length:
- 2196
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aggtggggac caaagtgagg aagagccgag ggaacgcagc caggcaggtg 121 gaatgggaac tctctggagc caagagagca ctccagaaga gaggctgccc gtggaaggga 181 gcaggccatg ggcagtggcc aggcgggtgc tgacagctat cctgattttg ggcctgctcc 241 tttgtttttc tgtgcttttg ttctacaact tccagaactg tggccctcgc ccctgtgaga 301 catctgtgtg tttggatctc cgggatcatt acctggcctc tgggaacaca agtgtggccc 361 cctgcaccga cttcttcagc tttgcctgtg gaagggccaa agagaccaat aattcttttc 421 aggagcttgc cacaaagaac aaaaaccgac ttcggagaat actggaggtc cagaattcct 481 ggcacccagg ctctggggag gagaaagcct tccagttcta caactcctgc atggatacac 541 ttgccattga agctgcaggg actggtcccc tcagacaagt tattgaggag cttggaggct 601 ggcgcatctc tggtaaatgg acttccttaa actttaaccg aacgctgaga cttctgatga 661 gtcagtatgg ccatttccct ttcttcagag cctacctagg acctcatcct gcctctccac 721 acacaccagt catccagata gaccagccag agtttgatgt tcccctcaag caagatcaag 781 aacagaagat ctatgcccag atctttcggg aatacctgac ttacctgaat cagctgggaa 841 ccttgctggg aggagaccca agcaaggtgc aagaacactc ttccttgtca atctccatca 901 cttcacggct gttccagttt ctgaggcccc tggagcagcg gcgggcacag ggcaagctct 961 tccagatggt cactatcgac cagctcaagg aaatggcccc cgccatcgac tggttgtcct 1021 gcttgcaagc gacattcaca ccgatgtccc tgagcccttc tcagtccctc gtggtccatg 1081 acgtggaata tttgaaaaac atgtcacaac tggtggagga gatgctgcta aagcagaggg 1141 actttctgca gagccacatg atcttagggc tggtggtgac cctttctcca gccctggaca 1201 gtcaattcca ggaggcacgc agaaagctca gccagaaact gcgggaactg acagagcaac 1261 cacccatgcc tgcccgccca cgatggatga agtgcgtgga ggagacaggc acgttcttcg 1321 agcccacgct ggcggctttg tttgttcgtg aggcctttgg cccgagcacc cgaagtgctg 1381 ccatgaaatt attcactgcg atccgggatg ccctcatcac tcgcctcaga aaccttccct 1441 ggatgaatga ggagacccag aacatggccc aggacaaggt tgctcaactg caggtggaga 1501 tgggggcttc agaatgggcc ctgaagccag agctggcccg acaagaatac aacgatatac 1561 agcttggatc gagcttcctg cagtctgtcc tgagctgtgt ccggtccctc cgagctagaa 1621 ttgtccagag cttcttgcag cctcaccccc aacacaggtg gaaggtgtcc ccttgggacg 1681 tcaatgctta ctattcggta tctgaccatg tggtagtctt tccagctgga ctcctccaac 1741 ccccattctt ccaccctggc tatcccagag ccgtgaactt tggcgctgct ggcagcatca 1801 tggcccacga gctgttgcac atcttctacc agctcTTACT GCCTGGGGGC TGCCTCGCCT 1861 GTGACAACCA TGCCCTCCAG GAAGCTCACC TGTGCCTGAA GCGCCATTAT GCTGCCTTTC 1921 CATTACCTAG CAGAACCTCC TTCAATGACT CCCTCACATT CTTAGAGAAT GCTGCAGACG 1981 TTGGGGGGCT AGCCATCGCG CTGCAGGCAT ACAGCAAGAG GCTGTTACGG CACCATGGGG 2041 AGACTGTCCT GCCCAGCCTG GACCTCAGCC CCCAGCAGAT CTTCTTTCGA AGCTATGCCC 2101 AGGTGATGTG TAGGAAGCCC AGCCCCCAGG ACTCTCACGA CACTCACAGC CCTCCACACC 2161 TCCGAGTCCA CGGGCCCCTC AGCAGCACCC CAGCCTTTGC CAGGTATTTC CGCTGTGCAC 2221 GTGGTGCTCT CTTGAACCCC TCCAGCCGCT GCCAGCTCTG GTACCCAACT TTCTTGTACA 2281 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2341 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2401 AGGACGATTA ACGAAAAACT ACGGATGGTG CACGCGTTAA GTCgacaatc aacctctgga 2461 ttacaaaatt tgtgaaagat t