Construct: ORF TRCN0000472038

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008868.1_s317c1
Derived from:
ccsbBroadEn_16013
DNA Barcode:
CATCCCAAACACCCTAGCGGAGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DOCK8 (81704)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472038
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81704 DOCK8 dedicator of cytokinesis 8 XM_011518049.2 33.9% 33.9% 1_2994del;3154C>T;3669G>A
2 human 81704 DOCK8 dedicator of cytokinesis 8 NM_001190458.2 25.6% 25.6% 1_4458del;4618C>T;5133G>A
3 human 81704 DOCK8 dedicator of cytokinesis 8 XM_011518045.3 25.6% 25.6% 1_4458del;4618C>T;5133G>A
4 human 81704 DOCK8 dedicator of cytokinesis 8 NM_001193536.1 25.2% 25.2% 1_4554del;4714C>T;5229G>A
5 human 81704 DOCK8 dedicator of cytokinesis 8 XM_011518047.3 25.2% 25.2% 1_4554del;4714C>T;5229G>A
6 human 81704 DOCK8 dedicator of cytokinesis 8 XM_011518048.2 25.2% 25.2% 1_4554del;4714C>T;5229G>A
7 human 81704 DOCK8 dedicator of cytokinesis 8 XM_017015173.1 25.2% 25.2% 1_4554del;4714C>T;5229G>A
8 human 81704 DOCK8 dedicator of cytokinesis 8 XM_011518046.2 24.9% 24.9% 1_4620del;4780C>T;5295G>A
9 human 81704 DOCK8 dedicator of cytokinesis 8 XM_017015174.1 24.9% 24.9% 1_4620del;4780C>T;5295G>A
10 human 81704 DOCK8 dedicator of cytokinesis 8 NM_203447.3 24.4% 24.4% 1_4758del;4918C>T;5433G>A
11 mouse 76088 Dock8 dedicator of cytokinesis 8 XM_006527435.3 22.2% 23.9% (many diffs)
12 mouse 76088 Dock8 dedicator of cytokinesis 8 XM_011247397.2 22.2% 23.9% (many diffs)
13 mouse 76088 Dock8 dedicator of cytokinesis 8 NM_028785.3 21.5% 23.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1605
ORF length:
1539
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca gatgactcct tttcccaccc aggtggagga acttctctgt aatctgaata
121gcatcttata tgacacagtg aaaatgaggg aatttcagga agatcctgag atgcttatgg
181atctcatgta cagaattgcc aagagttacc aggcatctcc tgatttgcgg ctgacctggc
241tccagaacat ggcagagaaa cacaccaaga agaagtgcta cacggaggct gccatgtgcc
301tggtgcacgc cgctgcgtta gtggctgagt atctgagcat gctggaggac cacagctacc
361tgcccgtggg cagtgtcagc ttccagaata tttcttccaa tgtgctggag gagtctgtgg
421tctctgagga caccctgtca cctgacgagg atggggtgtg cgcaggccag tacttcaccg
481agagtggcct ggtaggcctc ctggagcagg ccgcggagct cttcagcacg ggaggcttat
541atgagacagt taatgaggtc tacaagctgg tcatccccat cctagaagcg catcgagaat
601tccggaagct gacactcact cacagcaagc tgcagagagc cttcgacagc atcgttaaca
661aggatcataa gagaatgttt ggaacctact tccgagttgg tttctttgga tccaaatttg
721gggatttgga tgaacaggaa tttgtctaca aagagcctgc aattaccaag cttcctgaga
781tctcacatag actagaggca ttttatggtc aatgttttgg tgcagaattt gtggaagtga
841ttaaagactc cactcctgtg gacaaaacca agttggatcc taacaaggcc tacatacaga
901tcacttttgt ggagccctac tttgatgagt atgagatgaa agacagggtc acatactttg
961agaagaattt caacctccgg aggttcatgt acaccacccc gttcaccctg gaggggcggc
1021ctcggggaga gctgcatgag cagtacagaa ggaacacagt cctgaccact atgcacgcct
1081tcccctacat caagaccagg atcagcgTCA TCCAGAAGGA GGAGTTTGTT TTGACACCGA
1141TTGAAGTTGC CATTGAAGAC ATGAAGAAGA AGACCCTGCA GTTAGCAGTT GCCATTAACC
1201AGGAGCCGCC TGATGCAAAG ATGCTTCAGA TGGTGCTGCA AGGCTCTGTG GGAGCTACTG
1261TAAATCAGGG ACCACTGGAA GTAGCCCAAG TGTTTTTGGC TGAAATTCCT GCTGATCCAA
1321AACTCTATCG ACATCACAAC AAGTTGAGGT TATGCTTTAA GGAATTCATC ATGAGATGTG
1381GTGAAGCTGT AGAGAAAAAC AAGCGTCTCA TCACGGCAGA CCAGAGGGAA TATCAGCAGG
1441AACTCAAAAA GAACTATAAC AAGCTAAAAG AGAACCTCAG GCCAATGATC GAGCGGAAAA
1501TTCCAGAACT GTACAAGCCA ATATTCAGAG TTGAGAGTCA AAAGAGGGAC TCCTTCCACA
1561GATCTAGTTT CAGGAAATGT GAAACCCAGT TGTCACAGGG CAGCTACCCA ACTTTCTTGT
1621ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1681AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1741GAAAGGACGA CATCCCAAAC ACCCTAGCGG AGCCACGCGT TAAGTCgaca atcaacctct
1801ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)