Construct: ORF TRCN0000472043
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005108.1_s317c1
- Derived from:
- ccsbBroadEn_12917
- DNA Barcode:
- GCGCCACTCATTTACGTGGAATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SELENOI (85465)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472043
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85465 | SELENOI | selenoprotein I | NM_033505.4 | 64.9% | 61.9% | 729_820del;867_1191del |
| 2 | human | 85465 | SELENOI | selenoprotein I | NR_137633.2 | 9.7% | 1_90del;865_7974del | |
| 3 | mouse | 28042 | Selenoi | selenoprotein I | NM_027652.3 | 59.2% | 59.2% | (many diffs) |
| 4 | mouse | 28042 | Selenoi | selenoprotein I | NR_137634.1 | 8.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 840
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tggctacgaa tacgtgagcc cggagcagct ggctggcttt gataagtaca 121 agtacagtgc tgtggatacc aatccacttt ctctgtatgt catgcatcca ttctggaaca 181 ctatagtaaa ggtatttcct acttggctgg cgcccaatct gataactttt tctggctttc 241 tgctggtcgt attcaatttt ctgctaatgg catactttga tcctgacttt tatgcctcag 301 caccaggtca caagcacgtg cctgactggg tttggattgt agtgggcatc cTCAACTTCG 361 TAGCCTACAC TCTAGATGGT GTGGACGGAA AGCAAGCTCG CAGAACCAAT TCTAGCACTC 421 CCTTAGGGGA GCTTTTTGAT CATGGCCTGG ATAGTTGGTC ATGTGTTTAC TTTGTTGTGA 481 CTGTTTATTC CATCTTTGGA AGAGGATCAA CTGGTGTCAG TGTTTTTGTT CTTTATCTCC 541 TGCTATGGGT AGTTTTGTTT TCTTTCATCC TGTCCCACTG GGAAAAGTAT AACACAGGGA 601 TTCTTTTCCT GCCATGGGGA TATGACATTA GCCAGGTGAC TATTTCTTTT GTCTACATAG 661 TGACTGCAGT TGTGGGAGTT GAGGCCTGGT ATGAACCTTT CCTGTTTAAT TTCTTATATA 721 GAGACCTATT CACTGCAATG ATTATTGGTT GTGCATTATG TGTGACTCTT CCAATGAGTT 781 TATTAAACTT TTTCAGCGTG GATCCTTTGG TCACCTTCAG ATATTTTAGA GCTACATCCT 841 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGAGCGCC ACTCATTTAC GTGGAATCTA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt