Construct: ORF TRCN0000472044
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008683.1_s317c1
- Derived from:
- ccsbBroadEn_06594
- DNA Barcode:
- GTCAGCCCGTTAATGCGCGCCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MPZ (4359)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472044
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4359 | MPZ | myelin protein zero | NM_000530.8 | 95.9% | 95.7% | 0_1ins30;743A>C |
| 2 | human | 4359 | MPZ | myelin protein zero | XM_017001321.2 | 86.4% | 85.2% | (many diffs) |
| 3 | human | 4359 | MPZ | myelin protein zero | NM_001315491.2 | 76.9% | 95.7% | 0_1ins30;743A>C;745_936del |
| 4 | mouse | 17528 | Mpz | myelin protein zero | NM_001315499.1 | 87.7% | 89.9% | (many diffs) |
| 5 | mouse | 17528 | Mpz | myelin protein zero | NM_008623.5 | 87.7% | 89.9% | (many diffs) |
| 6 | mouse | 17528 | Mpz | myelin protein zero | NM_001315500.1 | 70.2% | 89.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 840
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ccgggcccct gcccctgccc cagctatggc tcctggggct ccctcatcca 121 gccccagccc tatcctggct gtgctgctct tctcttcttt ggtgctgtcc ccggcccagg 181 ccatcgtggt ttacaccgac agggaggtcc atggtgctgt gggctcccgg gtgaccctgc 241 actgctcctt ctggtccagt gagtgggtct cagatgacat ctccttcacc tggcgctacc 301 agcccgaagg gggcagagat gccatttcga tcttccacta tgccaaggga caacccTACA 361 TTGACGAGGT GGGGACCTTC AAAGAGCGCA TCCAGTGGGT AGGGGACCCT CGCTGGAAGG 421 ATGGCTCCAT TGTCATACAC AACCTAGACT ACAGTGACAA TGGCACGTTC ACTTGTGACG 481 TCAAAAACCC TCCAGACATA GTGGGCAAGA CCTCTCAGGT CACGCTGTAT GTCTTTGAAA 541 AAGTGCCAAC TAGGTACGGG GTCGTTCTGG GAGCTGTGAT CGGGGGTGTC CTCGGGGTGG 601 TGCTGTTGCT GCTGCTGCTT TTCTACGTGG TTCGGTACTG CTGGCTACGC AGGCAGGCGG 661 CCCTGCAGAG GAGGCTCAGT GCTATGGAGA AGGGGAAATT GCACAAGCCA GGAAAGGACG 721 CGTCGAAGCG CGGGCGGCAG ACGCCAGTGC TGTATGCAAT GCTGGACCAC AGCAGAAGCA 781 CCAAAGCTGT CAGTGAGAAG AAGGCCAAGG GGCTGGGGGA GTCTCGCAAG GATAAGACAT 841 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGAGTCAG CCCGTTAATG CGCGCCCTCA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt