Construct: ORF TRCN0000472079
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010815.1_s317c1
- Derived from:
- ccsbBroadEn_12406
- DNA Barcode:
- GCCGTGTAGCGTAACTGGAACGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCAR2 (57805)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472079
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57805 | CCAR2 | cell cycle and apoptosis re... | XM_011544604.2 | 41.3% | 41.3% | 1_1554del |
| 2 | human | 57805 | CCAR2 | cell cycle and apoptosis re... | NM_021174.6 | 39.5% | 39.5% | 1_1674del |
| 3 | human | 57805 | CCAR2 | cell cycle and apoptosis re... | NM_001363068.2 | 39.4% | 39.4% | 1_1674del;1989_1990insGCA |
| 4 | human | 57805 | CCAR2 | cell cycle and apoptosis re... | NM_001363069.2 | 39.4% | 39.4% | 1_1674del;1989_1990insGCA |
| 5 | human | 57805 | CCAR2 | cell cycle and apoptosis re... | NR_033902.2 | 23.1% | 1_1798del;2894_4728del | |
| 6 | mouse | 219158 | Ccar2 | cell cycle activator and ap... | NM_146055.3 | 33.8% | 35.1% | (many diffs) |
| 7 | mouse | 219158 | Ccar2 | cell cycle activator and ap... | XM_006518896.3 | 33.8% | 35.1% | (many diffs) |
| 8 | mouse | 219158 | Ccar2 | cell cycle activator and ap... | XM_006518897.3 | 33.7% | 35% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1161
- ORF length:
- 1095
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct actgagcctt cctgaaaagg tcgtgtcccc acctgaacct gagaaggagg 121 aggcggccaa ggaagaagcc accaaggagg aagaagccat caaagaggag gtggtcaagg 181 agcccaagga tgaggcacag aatgagggcc cggctacaga gtcagaggcc ccgctgaagg 241 aggatgggct tttgcccaaa ccactctctt ctgggggaga ggaagaagaa aaaccccggg 301 gcgaggcttc tgaggacctg tgtgagatgg ccctggaccc agaactgttg cttctgaggg 361 atgatggaga ggaggagttt gcaggagcaa agctggagga ttcggaggtc cggtccgttg 421 cctcaaacca gtcagagatg gagttctctt cacttcagga catgcccaag gagctggatc 481 cctctgctgt gctcccctta gactgtctgc ttgcttttgt gttctttgat gccaactggt 541 gtggctactt gcaccggcga gacttagaga ggatcctcct tacccttggg atccggctca 601 gtgcagagca ggccaagcag ctggtcagca gggtggtgac ccagaacatc tgccagtacc 661 ggagccttca gtacagccgc caggagggcc tggatggtgg ccttcccgag gaggtgctct 721 tcggaaacct ggacctgctg ccccctcctg ggaaaagcac gaagccaggt gctgccccca 781 cagaacacaa agccttggtg tcccacaatg gcagcctgat taacgtgggg agccTGCTGC 841 AGCGCGCGGA GCAGCAGGAC AGCGGCCGGC TCTACCTAGA GAACAAGATC CACACACTGG 901 AGCTGAAGCT GGAGGAGAGC CATAACCGTT TCTCAGCCAC TGAAGTAACC AATAAGACGC 961 TGGCGGCAGA GATGCAGGAG CTGCGAGTCC GGCTGGCGGA GGCCGAGGAG ACCGCCCGGA 1021 CGGCGGAGCG ACAGAAGAGC CAGCTCCAGC GGCTGCTGCA GGAGCTCCGC AGGCGTCTGA 1081 CCCCCCTGCA GCTGGAGATC CAGCGGGTGG TGGAAAAGGC TGACAGCTGG GTGGAGAAGG 1141 AGGAGCCGGC ACCTAGCAAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1201 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1261 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCCG TGTAGCGTAA 1321 CTGGAACGGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt