Construct: ORF TRCN0000472116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017160.1_s317c1
- Derived from:
- ccsbBroadEn_06552
- DNA Barcode:
- CAAACACCCAATCCGTACCTTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGEA4 (4103)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472116
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_001011548.1 | 99.8% | 99.6% | 517G>A |
| 2 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_001011549.1 | 99.8% | 99.6% | 517G>A |
| 3 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_001011550.1 | 99.8% | 99.6% | 517G>A |
| 4 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_002362.4 | 99.8% | 99.6% | 517G>A |
| 5 | human | 4103 | MAGEA4 | MAGE family member A4 | XM_005274678.3 | 99.8% | 99.6% | 517G>A |
| 6 | human | 4103 | MAGEA4 | MAGE family member A4 | XM_005274679.4 | 99.8% | 99.6% | 517G>A |
| 7 | human | 4100 | MAGEA1 | MAGE family member A1 | NM_004988.5 | 85.2% | 75.3% | (many diffs) |
| 8 | human | 4103 | MAGEA4 | MAGE family member A4 | XM_005274677.2 | 79.7% | 79.5% | 1_240del;757G>A |
| 9 | human | 100533997 | MAGEA10-MAGEA5 | MAGEA10-MAGEA5 readthrough | NM_001204811.3 | 34.5% | 30.5% | (many diffs) |
| 10 | human | 4104 | MAGEA5 | MAGE family member A5 | NM_021049.4 | 34.5% | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1017
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttctgagcag aagagtcagc actgcaagcc tgaggaaggc gttgaggccc 121 aagaagaggc cctgggcctg gtgggtgcac aggctcctac tactgaggag caggaggctg 181 ctgtctcctc ctcctctcct ctggtccctg gcaccctgga ggaagtgcct gctgctgagt 241 cagcaggtcc tccccagagt cctcagggag cctctgcctt acccactacc atcagcttca 301 cttgctggag gcaacccaat gagggttcca gcagccaaga agaggagggg ccaagcacct 361 cgcctgacgc agagtccttg ttccgagaag cactcagtaa caaggtggat gagttggctc 421 attttctgct ccgcaagtat cgagccaagg agctggtcac aaaggcagaa atgctggaga 481 gagtcatcaa aaattacaag cgctgctttc ctgtgatctt cggcaaagcc tccgagtccc 541 tgaagatgat ctttggcatt gacgtgaagg aagtggaccc caccagcaac acctacaccc 601 ttgtcacctg cctgggcctt tcctatgatg gcctgctggg taataatcag atctttccca 661 agacaggccT TCTGATAATC GTCCTGGGCA CAATTGCAAT GGAGGGCGAC AGCGCCTCTG 721 AGGAGGAAAT CTGGGAGGAG CTGGGTGTGA TGGGGGTGTA TGATGGGAGG GAGCACACTG 781 TCTATGGGGA GCCCAGGAAA CTGCTCACCC AAGATTGGGT GCAGGAAAAC TACCTGGAGT 841 ACCGGCAGGT ACCCGGCAGT AATCCTGCGC GCTATGAGTT CCTGTGGGGT CCAAGGGCTC 901 TGGCTGAAAC CAGCTATGTG AAAGTCCTGG AGCATGTGGT CAGGGTCAAT GCAAGAGTTC 961 GCATTGCCTA CCCATCCCTG CGTGAAGCAG CTTTGTTAGA GGAGGAAGAG GGAGTCTGCC 1021 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1081 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1141 ATATATCTTG TGGAAAGGAC GACAAACACC CAATCCGTAC CTTTCCACGC GTTAAGTCga 1201 caatcaacct ctggattaca aaatttgtga aagatt