Construct: ORF TRCN0000472121
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010882.1_s317c1
- Derived from:
- ccsbBroadEn_01718
- DNA Barcode:
- CGTGGCCTAATTTCCCAGTTTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSNAX (7257)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472121
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7257 | TSNAX | translin associated factor X | NM_005999.3 | 100% | 100% | |
| 2 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028399.1 | 29.5% | (many diffs) | |
| 3 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028395.1 | 21.7% | (many diffs) | |
| 4 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028397.1 | 21.2% | (many diffs) | |
| 5 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028394.1 | 21.1% | (many diffs) | |
| 6 | mouse | 53424 | Tsnax | translin-associated factor X | NM_016909.2 | 85.7% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 936
- ORF length:
- 870
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caacaaagaa ggatcaggag ggttcaggaa aaggaagcat gacaatttcc 121 cacataacca aagaagagaa gggaaggatg ttaattcatc ttcacccgtg atgttggcct 181 ttaaatcatt tcagcaggaa cttgatgcaa ggcatgacaa atatgagaga cttgtgaaac 241 ttagtcggga tataactgtt gaaagtaaaa ggacaatttt tctcctccat aggattacaa 301 gtgctcctga tatggaagat atattgactg aatcagaaat taaattggat ggtgtcagac 361 aaaagatatt ccaggtagcc caagagctat caggggaaga tatgcatcag ttccatcgag 421 ccattactac aggactacag gaatatgtgg aagctgtctc ttttcaacac ttcatcaaaa 481 cacgatcatt aattagtatg gatgaaatta ataaacaatt gatatttacg actgaagaca 541 atgggaaaga aaataaaact ccctcctcTG ATGCACAGGA TAAGCAGTTT GGTACTTGGA 601 GACTGAGAGT CACACCTGTC GATTACCTTC TGGGAGTGGC TGACTTAACT GGAGAATTGA 661 TGCGGATGTG TATTAACAGT GTGGGGAATG GGGACATTGA TACCCCCTTT GAAGTGAGCC 721 AGTTTTTACG TCAGGTTTAT GATGGGTTTT CATTCATTGG CAACACTGGA CCTTACGAGG 781 TTTCTAAGAA GCTGTATACC TTGAAACAAA GTTTGGCCAA AGTGGAGAAT GCTTGTTATG 841 CCTTGAAAGT CAGAGGGTCA GAAATTCCAA AACATATGTT GGCAGATGTG TTTTCAGTTA 901 AAACAGAAAT GATAGATCAA GAAGAGGGCA TTTCTTACCC AACTTTCTTG TACAAAGTGG 961 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1021 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1081 ACGTGGCCTA ATTTCCCAGT TTGCCACGCG TTAAGTCgac aatcaacctc tggattacaa 1141 aatttgtgaa agatt