Construct: ORF TRCN0000472129
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017873.1_s317c1
- Derived from:
- ccsbBroadEn_03651
- DNA Barcode:
- TATAAATTGAGAACTGTCATTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NGLY1 (55768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472129
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55768 | NGLY1 | N-glycanase 1 | NM_018297.4 | 100% | 100% | |
| 2 | human | 55768 | NGLY1 | N-glycanase 1 | NM_001145293.1 | 94.4% | 93.3% | (many diffs) |
| 3 | human | 55768 | NGLY1 | N-glycanase 1 | NM_001145294.1 | 93.5% | 93.4% | 0_1ins112;3_4insCTGACAACATCCTC |
| 4 | human | 55768 | NGLY1 | N-glycanase 1 | XM_005265316.1 | 91.5% | 91.5% | 1257_1258ins165 |
| 5 | human | 55768 | NGLY1 | N-glycanase 1 | XM_011533944.1 | 88.2% | 88.2% | 0_1ins231 |
| 6 | human | 55768 | NGLY1 | N-glycanase 1 | XM_017006839.2 | 86.8% | 84.1% | (many diffs) |
| 7 | human | 55768 | NGLY1 | N-glycanase 1 | NM_001145295.1 | 85.3% | 81.5% | 1610_1611ins178;1674_1675ins110 |
| 8 | human | 55768 | NGLY1 | N-glycanase 1 | XR_940471.2 | 85.3% | 1_53del;1053_1144del;2108_2300del | |
| 9 | human | 55768 | NGLY1 | N-glycanase 1 | XR_002959548.1 | 82.2% | 1_53del;1054_1055ins146;1870_2063del | |
| 10 | human | 55768 | NGLY1 | N-glycanase 1 | XM_005265317.1 | 76.9% | 73.2% | 1257_1258ins165;1445_1446ins178;1509_1510ins110 |
| 11 | human | 55768 | NGLY1 | N-glycanase 1 | XR_001740200.2 | 68.4% | (many diffs) | |
| 12 | mouse | 59007 | Ngly1 | N-glycanase 1 | NM_021504.3 | 85.3% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2028
- ORF length:
- 1962
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcggca ttgggcagct cctcaggctc ggcgtccccg gccgtggctg 121 agctctgcca gaacaccccg gagacctttt tggaggcctc caagctgctg ctcacctatg 181 ctgacaacat cctcagaaac cctaatgatg aaaaatatag atccatccgg attggaaaca 241 cagccttttc tactagactc ttgcctgtca gaggagctgt tgaatgttta tttgaaatgg 301 gctttgaaga gggagaaaca catctcatct ttcctaaaaa agcttcagtg gagcagctgc 361 aaaaaattcg tgacctgatt gccatagaga gaagtagcag actggatggc tcaaataaga 421 gccacaaagt aaagtcatct cagcaacctg cagccagtac ccagcttcct acaacaccat 481 cttcaaatcc cagtgggtta aaccagcaca caaggaaccg tcaagggcag tcatcagatc 541 caccatctgc ttcaacggtt gctgctgact cagccattct agaagttctt cagtccaaca 601 ttcagcatgt gctggtctat gaaaatcctg ctcttcagga gaaagcgttg gcttgtattc 661 cggtccaaga actaaaaagg aaatcacaag aaaagttatc gagagctaga aaattggata 721 aaggtatcaa tataagtgat gaggattttc ttttgctgga gcttttgcac tggtttaagg 781 aagaattttt tcactgggtg aataacgttt tgtgcagcaa atgtggtgga cagactaggt 841 ctagagatag atcattactg cccagtgatg atgagctgaa gtggggtgca aaggaagtgg 901 aagatcatta ctgtgatgcc tgccagttca gcaatcgatt cccaagatat aataaccctg 961 agaaactttt ggaaacaaga tgtggacggt gtggcgagtg ggccaattgt tttacactgt 1021 gctgccgagc tgtagggttt gaagctcgct atgtttggga ttacacagac catgtctgga 1081 cagaagtcta ttctccttct cagcagcggt ggctgcactg tgatgcatgt gaagatgtct 1141 gtgacaagcc actcctttat gaaataggat ggggcaagaa gctttcctat gtcatagcat 1201 tttcaaaaga tgaggtagtt gatgtcactt ggcgatattc ctgcaaacat gaagaggtga 1261 ttgccagaag aactaaggtt aaagaagcat tacttcgaga cactattaat gggcttaata 1321 agcagaggca actgtttttg tcagaaaaca gaaggaaaga acttctccag aggataattg 1381 tggagcttgt tgaatttata tctcccaaaa cccctaaacc tggagaactt gggggaagaa 1441 tatctgggtc agtggcttgg agagtagccc gaggtgaaat gggtctacag agaaaagaaa 1501 ccttgtttat tccctgtgaa aatgagaaga tttctaaaca gctccacctt tgttacaata 1561 ttgtgaaaga tcgttatgtt cgagtttcaa ataacaatca aaccatttct ggatGGGAGA 1621 ATGGCGTGTG GAAAATGGAA TCTATATTCA GAAAAGTTGA AACAGACTGG CACATGGTAT 1681 ATTTGGCCCG AAAGGAAGGA TCATCTTTTG CTTATATTTC CTGGAAGTTT GAGTGTGGGT 1741 CAGTTGGCCT AAAAGTAGAT AGCATTTCTA TTAGAACAAG TAGTCAAACT TTTCAGACTG 1801 GAACAGTAGA ATGGAAATTG CGATCTGATA CAGCACAAGT AGAACTGACA GGCGATAACA 1861 GTCTTCACTC CTATGCTGAT TTTTCTGGTG CCACTGAAGT TATTTTGGAA GCAGAATTAA 1921 GCAGAGGAGA TGGTGATGTC GCTTGGCAAC ACACCCAGCT GTTTAGACAA AGCTTAAATG 1981 ACCATGAAGA AAATTGTTTG GAGATAATTA TAAAATTCAG TGACCTTTGC CCAACTTTCT 2041 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2101 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2161 GTGGAAAGGA CGATATAAAT TGAGAACTGT CATTTCCACG CGTTAAGTCg acaatcaacc 2221 tctggattac aaaatttgtg aaagatt