Construct: ORF TRCN0000472131
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001596.1_s317c1
- Derived from:
- ccsbBroadEn_11358
- DNA Barcode:
- GCACTTCGATGTTCCGATGCACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B4GALT6 (9331)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472131
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9331 | B4GALT6 | beta-1,4-galactosyltransfer... | NM_004775.4 | 89.7% | 89.7% | 470_586del |
| 2 | human | 9331 | B4GALT6 | beta-1,4-galactosyltransfer... | XM_005258387.4 | 80.9% | 75.9% | (many diffs) |
| 3 | human | 9331 | B4GALT6 | beta-1,4-galactosyltransfer... | XM_006722578.3 | 79.8% | 79.5% | 232_233ins114;356_472del |
| 4 | human | 9331 | B4GALT6 | beta-1,4-galactosyltransfer... | NM_001330570.2 | 79.5% | 79.5% | 114_115ins117;353_469del |
| 5 | human | 9331 | B4GALT6 | beta-1,4-galactosyltransfer... | XM_006722579.4 | 78.2% | 78.2% | 0_1ins132;338_454del |
| 6 | human | 9331 | B4GALT6 | beta-1,4-galactosyltransfer... | XM_017026089.2 | 78.2% | 78.2% | 0_1ins132;338_454del |
| 7 | human | 9331 | B4GALT6 | beta-1,4-galactosyltransfer... | XM_017026090.1 | 70.5% | 57.6% | (many diffs) |
| 8 | human | 9331 | B4GALT6 | beta-1,4-galactosyltransfer... | XM_017026091.1 | 70% | 53.5% | (many diffs) |
| 9 | human | 9331 | B4GALT6 | beta-1,4-galactosyltransfer... | XM_017026092.2 | 58.3% | 55.6% | (many diffs) |
| 10 | mouse | 56386 | B4galt6 | UDP-Gal:betaGlcNAc beta 1,4... | NM_019737.2 | 79% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1095
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtgctcagg cggatgatgc gggtttccaa tcgctctctc ctcgccttca 121 tcttcttctt ctccctctct tcgtcctgtc tgtacttcat ctatgtggcc ccaggcatcg 181 ccaacacata tctctttatg gtacaagctc gaggtataat gttgagagaa aatgtgaaaa 241 caataggtca tatgatcagg ctgtacacaa ataaaaacag tacgctcaac ggtacagatt 301 atcccgaagg caataattca agtgattatc ttgttcaaac aacaacgtat ctcccggaaa 361 acttcacata ctcaccatac ctcccctgtc cagaaaagct gccttatatg cgaggattcc 421 tcaatgtcaa tgtaagcgaa gtcagttttg atgaaattca tcaactcttc tccaaggatt 481 tagatattga gccagggggt cattggaggc caaaagactg taaacccaga tggaagactg 541 gcacacaacc ttttaaccgt gcgatgcttt tcaatgtggg cttcaaagag gccatgaaag 601 acagtgtctg ggactgtgta atcttccacg atgtggatca tcTACCTGAA AATGACCGGA 661 ACTATTACGG ATGTGGAGAA ATGCCACGTC ATTTTGCTGC AAAGCTGGAT AAATACATGT 721 ATATTCTTCC ATATAAAGAA TTTTTTGGTG GTGTAAGTGG GCTGACAGTG GAACAATTTA 781 GAAAGATCAA TGGTTTTCCT AATGCCTTCT GGGGATGGGG AGGAGAAGAT GATGACCTTT 841 GGAACAGAGT TCACTATGCT GGATATAATG TAACCAGACC AGAGGGAGAC TTAGGAAAAT 901 ACAAGTCAAT TCCTCATCAC CATAGAGGTG AAGTCCAGTT TTTAGGACGG TATAAATTAC 961 TAAGGTATTC CAAGGAGCGT CAGTACATCG ATGGACTGAA CAATTTAATA TATAGGCCAA 1021 AAATACTGGT TGATAGGTTG TATACAAACA TATCTGTAAA CCTCATGCCA GAGTTAGCTC 1081 CAATCGAAGA CTATTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1141 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1201 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCACTTCGAT GTTCCGATGC 1261 ACAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt