Construct: ORF TRCN0000472137
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007748.1_s317c1
- Derived from:
- ccsbBroadEn_00434
- DNA Barcode:
- CAGCTGTCGGACACTTCTATCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEFA1 (1667)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472137
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 728358 | DEFA1B | defensin alpha 1B | NM_001042500.1 | 100% | 100% | |
| 2 | human | 1667 | DEFA1 | defensin alpha 1 | NM_004084.3 | 100% | 100% | |
| 3 | human | 1668 | DEFA3 | defensin alpha 3 | NM_005217.4 | 99.6% | 98.9% | 194A>C |
| 4 | human | 728358 | DEFA1B | defensin alpha 1B | NM_001302265.1 | 93% | 93% | 1_21del |
| 5 | human | 1667 | DEFA1 | defensin alpha 1 | XM_011534740.3 | 93% | 93% | 1_21del |
| 6 | human | 1668 | DEFA3 | defensin alpha 3 | XM_011534741.2 | 92.7% | 92% | 1_21del;215A>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 372
- ORF length:
- 282
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAGGACCCT CGCCATCCTT GCTGCCATTC 121 TCCTGGTGGC CCTGCAGGCC CAGGCTGAGC CACTCCAGGC AAGAGCTGAT GAGGTTGCTG 181 CAGCCCCGGA GCAGATTGCA GCGGACATCC CAGAAGTGGT TGTTTCCCTT GCATGGGACG 241 AAAGCTTGGC TCCAAAGCAT CCAGGCTCAA GGAAAAACAT GGCCTGCTAT TGCAGAATAC 301 CAGCGTGCAT TGCAGGAGAA CGTCGCTATG GAACCTGCAT CTACCAGGGA AGACTCTGGG 361 CATTCTGCTG CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 421 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 481 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAG CTGTCGGACA CTTCTATCAC 541 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t