Construct: ORF TRCN0000472142
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012366.1_s317c1
- Derived from:
- ccsbBroadEn_11528
- DNA Barcode:
- CTGTCATCTCTGGACGTGGCCGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MXD4 (10608)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472142
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10608 | MXD4 | MAX dimerization protein 4 | NM_006454.3 | 27.1% | 26.3% | (many diffs) |
| 2 | mouse | 17122 | Mxd4 | Max dimerization protein 4 | NM_010753.2 | 25.5% | 24.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 213
- ORF end:
- 387
- ORF length:
- 174
- Sequence:
-
1 TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA 61 CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA 121 GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GCTAGCATCG 181 ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCATGGAGCT GAACTCCCTG CTGATCCTGC 241 TGGAGGCGGC CGAGTACCTG GAGCGCAGGG ATCGAGAGGC CGAGCACGGC TACGCCTCGG 301 TGCTGCCCTT CGACGGCGAC TTCGCCAGGG AGAAAACAAA GGCGGCCGGC CTGGTGCGCA 361 AGGCCCCGAA CAACAGGCCT CCTCAGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTGTCATC 541 TCTGGACGTG GCCGCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 601 aagatt