Construct: ORF TRCN0000472149
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002917.1_s317c1
- Derived from:
- ccsbBroadEn_09377
- DNA Barcode:
- CAAACTACACGGGACCCTTGACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFT43 (112752)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472149
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112752 | IFT43 | intraflagellar transport 43 | NM_052873.3 | 99.8% | 99.5% | 280G>A |
| 2 | human | 112752 | IFT43 | intraflagellar transport 43 | NM_001102564.1 | 89.1% | 85.5% | (many diffs) |
| 3 | human | 112752 | IFT43 | intraflagellar transport 43 | NR_045665.1 | 55.1% | (many diffs) | |
| 4 | human | 112752 | IFT43 | intraflagellar transport 43 | NR_045664.1 | 52% | 1_34del;249_250ins95;579_951del | |
| 5 | human | 112752 | IFT43 | intraflagellar transport 43 | NM_001255995.2 | 47.6% | 32.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggatttgctc gacttggacg aggagcttcg ctacagcttg gctacctcca 121 gggccaagat gggtcgccga gctcaacagg agtcagcgca ggccgagaat cacctcaatg 181 gcaagaattc ctctttgact ctgactggag agacttccTC TGCTAAATTA CCTCGCTGCC 241 GACAGGGAGG CTGGGCAGGT GATTCCGTGA AGGCTTCGAA CGGTACCCAA ACAGGCAAAC 301 AACAGCTGGA TCTGAACGCA TGCTATCACA AAACGCATCA CAGAAATTTG GGGCTGGCTT 361 CATTGGAAGA GGCAGATATT CCTATCATTC CGGATCTGGA GGAAGTACAG GAAGAAGACT 421 TTGTTTTGCA GGTGGCAGCC CCTCCCAGCA TCCAGATAAA GCGGGTGATG ACCTACCGTG 481 ACCTGGACAA TGACCTCATG AAGTACTCAG CCATTCAGAC ACTGGATGGG GAGATCGACC 541 TGAAACTCCT CACCAAAGTG CTCGCGCCGG AGCACGAAGT CCGGGAGGAT GATGTCGGCT 601 GGGACTGGGA CCATCTGTTC ACTGAGGTGT CCTCAGAGGT CCTCACTGAG TGGGACCCAC 661 TGCAGACGGA GAAGGAGGAC CCTGCGGGGC AGGCCAGGCA CACCTGCCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA CAAACTACAC GGGACCCTTG ACATACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt