Construct: ORF TRCN0000472156
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001420.1_s317c1
- Derived from:
- ccsbBroadEn_03583
- DNA Barcode:
- CCACAGCTCGAGACCGTATTTGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DRAM1 (55332)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472156
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55332 | DRAM1 | DNA damage regulated autoph... | NM_018370.3 | 100% | 100% | |
| 2 | human | 55332 | DRAM1 | DNA damage regulated autoph... | XM_005269004.2 | 86.9% | 86.9% | 577_578ins93 |
| 3 | human | 55332 | DRAM1 | DNA damage regulated autoph... | XM_017019578.1 | 75.6% | 75.6% | 0_1ins174 |
| 4 | human | 55332 | DRAM1 | DNA damage regulated autoph... | XM_005269005.2 | 66.8% | 66.8% | 340_341ins237 |
| 5 | mouse | 71712 | Dram1 | DNA-damage regulated autoph... | NM_027878.2 | 88.3% | 94.5% | (many diffs) |
| 6 | mouse | 71712 | Dram1 | DNA-damage regulated autoph... | XM_011243576.1 | 65.9% | 70.1% | (many diffs) |
| 7 | mouse | 71712 | Dram1 | DNA-damage regulated autoph... | XM_017314100.1 | 65.9% | 70.1% | (many diffs) |
| 8 | mouse | 71712 | Dram1 | DNA-damage regulated autoph... | XR_380446.3 | 46.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtgcttcctg aggggaatgg ctttcgtccc cttcctcttg gtgacctggt 121 cgtcagccgc cttcattatc tcctacgtgg tcgccgtgct ctccgggcac gtcaacccct 181 tcctcccgta tatcagtgat acgggaacaa cacctccaga gagtggtatt tttggattta 241 tgataaactt ctctgcattt cttggtgcag ccacgatgta tacaagatac aaaatagtac 301 agaagcaaaa tcaaacctgc tattTCAGCA CTCCTGTTTT TAACTTGGTG TCTTTAGTGC 361 TTGGATTGGT GGGATGTTTC GGAATGGGCA TTGTCGCCAA TTTTCAGGAG TTAGCTGTGC 421 CAGTGGTTCA TGACGGGGGC GCTCTTTTGG CCTTTGTCTG TGGTGTCGTG TACACGCTCC 481 TACAGTCCAT CATCTCTTAC AAATCATGTC CCCAGTGGAA CAGTCTCTCG ACATGCCACA 541 TACGGATGGT CATCTCTGCC GTTTCTTGCG CAGCTGTCAT CCCCATGATT GTCTGTGCTT 601 CACTAATTTC CATAACCAAG CTGGAGTGGA ATCCAAGAGA AAAGGATTAT GTATATCACG 661 TAGTGAGTGC GATCTGTGAA TGGACAGTGG CCTTTGGTTT TATTTTCTAC TTCCTAACTT 721 TCATCCAAGA TTTCCAGAGT GTCACCCTAA GGATATCCAC AGAAATCAAT GGTGATATTT 781 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 841 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 901 TTTATATATC TTGTGGAAAG GACGACCACA GCTCGAGACC GTATTTGCTA CGCGTTAAGT 961 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt