Transcript: Mouse NM_027878.2

Mus musculus DNA-damage regulated autophagy modulator 1 (Dram1), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Dram1 (71712)
Length:
2807
CDS:
154..870

Additional Resources:

NCBI RefSeq record:
NM_027878.2
NBCI Gene record:
Dram1 (71712)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_027878.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000180557 GCAATCGATCATCTCCTACAA pLKO.1 570 CDS 100% 4.950 6.930 N Dram1 n/a
2 TRCN0000183921 CCAGAGTGTCACTCTAAGGAT pLKO.1 822 CDS 100% 3.000 2.400 N Dram1 n/a
3 TRCN0000329081 CCAGAGTGTCACTCTAAGGAT pLKO_005 822 CDS 100% 3.000 2.400 N Dram1 n/a
4 TRCN0000329084 TTGCCTGTGCTTCACTCATTT pLKO_005 677 CDS 100% 13.200 9.240 N Dram1 n/a
5 TRCN0000329085 ATCGATCATCTCCTACAAATC pLKO_005 573 CDS 100% 10.800 7.560 N Dram1 n/a
6 TRCN0000329082 TAGTGATTATATACCCTAATG pLKO_005 1348 3UTR 100% 10.800 7.560 N Dram1 n/a
7 TRCN0000329023 TGATAAACTTCTCTGCATTTC pLKO_005 329 CDS 100% 10.800 7.560 N Dram1 n/a
8 TRCN0000179902 CTGTGCTTCACTCATTTCTAT pLKO.1 681 CDS 100% 5.625 3.938 N Dram1 n/a
9 TRCN0000180983 CAAGCTGGAATGGAATCCAAA pLKO.1 705 CDS 100% 4.950 3.465 N Dram1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_027878.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03583 pDONR223 100% 88.3% 94.5% None (many diffs) n/a
2 ccsbBroad304_03583 pLX_304 0% 88.3% 94.5% V5 (many diffs) n/a
3 TRCN0000472156 CCACAGCTCGAGACCGTATTTGCT pLX_317 63.4% 88.3% 94.5% V5 (many diffs) n/a
4 ccsbBroadEn_12204 pDONR223 100% 48% 51.6% None (many diffs) n/a
5 ccsbBroad304_12204 pLX_304 0% 48% 51.6% V5 (many diffs) n/a
6 TRCN0000472687 TATCTTTATCAGCTCGATGGCCGC pLX_317 95.6% 48% 51.6% V5 (many diffs) n/a
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