Construct: ORF TRCN0000472163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016507.1_s317c1
- Derived from:
- ccsbBroadEn_03077
- DNA Barcode:
- TAATTCTATATAGTGAACGACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGALSL (29094)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472163
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29094 | LGALSL | galectin like | NM_014181.3 | 100% | 100% | |
| 2 | mouse | 216551 | Lgalsl | lectin, galactoside binding... | NM_173752.4 | 91.6% | 99.4% | (many diffs) |
| 3 | mouse | 216551 | Lgalsl | lectin, galactoside binding... | XM_006514629.3 | 86.6% | 92.4% | (many diffs) |
| 4 | mouse | 102632642 | Lgals1-ps2 | lectin, galactose binding, ... | XR_875478.2 | 10.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gGGATCAGTG GCCGACAGCG ATGCCGTGGT GAAACTAGAT GATGGCCATT 121 TAAACAACTC TTTGAGCTCT CCAGTTCAAG CGGACGTGTA CTTCCCACGA CTGATAGTTC 181 CATTTTGTGG GCACATTAAA GGTGGCATGA GACCAGGCAA GAAGGTGTTA GTGATGGGCA 241 TCGTAGACCT CAACCCAGAG AGCTTTGCAA TCAGCTTGAC CTGTGGGGAC TCAGAAGACC 301 CTCCTGCCGA TGTGGCAATC GAACTCAAAG CTGTGTTCAC AGATCGGCAG CTACTCAGAA 361 ATTCTTGTAT ATCTGGGGAG AGGGGTGAAG AACAGTCAGC AATCCCTTAC TTTCCATTCA 421 TTCCAGACCA GCCATTCAGG GTGGAAATTC TTTGTGAGCA CCCACGTTTC CGAGTGTTTG 481 TGGATGGACA CCAACTTTTT GATTTTTACC ATCGCATTCA AACGTTATCT GCAATTGACA 541 CCATAAAGAT AAATGGAGAC CTCCAGATCA CCAAGCTTGG CTGCCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGATAA TTCTATATAG TGAACGACCC CACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t