Construct: ORF TRCN0000472164
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004814.1_s317c1
- Derived from:
- ccsbBroadEn_13412
- DNA Barcode:
- CTGCAGGGATGTGGGGGCACAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM124A (220108)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472164
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220108 | FAM124A | family with sequence simila... | NM_001242312.2 | 100% | 100% | |
| 2 | human | 220108 | FAM124A | family with sequence simila... | NM_145019.4 | 93.8% | 93.6% | 101_208del |
| 3 | human | 220108 | FAM124A | family with sequence simila... | XM_017020419.2 | 53.6% | 51.1% | (many diffs) |
| 4 | human | 220108 | FAM124A | family with sequence simila... | NM_001330522.1 | 53.3% | 52% | (many diffs) |
| 5 | human | 220108 | FAM124A | family with sequence simila... | XM_011534978.2 | 50.3% | 47.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1704
- ORF length:
- 1638
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cccaaaggcg ggcggcggcg gcgaggagga cgactgcgtg gactcgggcg 121 ccgagaccgg agggtccgac tacagccacc tgtcctccac gagcagtgag ctttccgttg 181 aagaggcgca ggaccctttc ctggtcagca tccacataat cgcagaccca ggggagtccc 241 agcccctgca ggaggccatc gacaacgtcc tggcgtggat ccaccccgac ctcccgctgt 301 tccgggtgtc cgagaggcgg gcgtcccggc ggcggcggaa gccccccaag ggcgctcagc 361 cagcgctggc tgtggtgctg ttcctgcagg aggagtacgg cgaagagcag atcctgcagc 421 tgcaccgcac actgcagcag ccgccctggc gccaccacca caccgagcag gtgcacggcc 481 ggttcctgcc ctacctgccc tgcagccagg acttcttcac gctggcccct gggacgccgc 541 tttgggccat ccggcccgtg cactacggca aggaaatcgt gcgcttcacc gtctactgtc 601 gctacgacaa ctatgctgac agcctcaggt tctaccagct gattctccgg aggagcccca 661 gccagaagaa agcggacttc tgcatcttcc ctattttttc caacctggat gtggacatcc 721 agttctccct gaaaagactg ccctgtgacc agtgcccggt gcccaccgac tcctccgtgc 781 tggagttccg agtgagggac ataggcgagc tcgtgcctct cctgcccaac ccttgcagcc 841 ccatcagcga ggggcgctgg cagacggagg accatgatgg gaacaagatc ctcctacagg 901 cacaaagggt gcataagaag tttcctaaac ctggcagagt acatcatgcc tccgagaaga 961 aacgtcattc cactcctttg ccgagcactg ctgtaccaag ccatacacct ggcagcagcc 1021 agcagtcccc gctcaacagt cctcacccgg ggcccatccg gacaggcctg cctcctgggc 1081 accagcagga atttgccgga cgagccaaca gcacccccaa ccctccctgg tctttccaga 1141 gaagcaagtc cttgttttgt ttgcccacgg gaggcccctc cctggcctcc tcagctgaac 1201 cacagtggtt ttcaaacaca ggtgccccag ggcacagggc atcagagtgg aggcatggcc 1261 accTCCTCTC CATCGATGAC CTAGAGGGGG CCCAGGAGAC AGACGTGGAC ACAGGCCTGC 1321 GGCTGTCCTC ATCGGACCTG TCTGTGGTCT CTGCATATTC TGCACCCAGT AGGTTCTGCA 1381 GCACAGTGGA GACACCCCTC CCCTCCGAAA GATGCAGCAG CCACTGGGCA GCTCACAAGG 1441 ATTCCAGGGA GGGACCACTG CCCACTGTCA GCAGGGTGAC CACAGAGGCC TCCTGGGCTT 1501 CCCTCCCTTT CTTCACCAAA AGGTCTTCCA GCTCCTCAGC GACAGCTCGT GCTGCTCCCC 1561 CAGCTCCCAG CACCTCCACC CTCACAGACT CCTCCCCACA GCTCCCATGC GATACCCCCA 1621 AAGTCAAGCA GACTGATGGA GACATGCCAC CACCCCCAGG GTCGGCTGGC CCCGGGGATA 1681 ACGACATGGA GGAATTCTAC ATCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1741 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1801 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TGCAGGGATG 1861 TGGGGGCACA ATTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1921 att