Construct: ORF TRCN0000472177
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017433.1_s317c1
- Derived from:
- ccsbBroadEn_07312
- DNA Barcode:
- CGAGCACTATGCGCTCTGTGTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALKBH1 (8846)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472177
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8846 | ALKBH1 | alkB homolog 1, histone H2A... | NM_006020.3 | 99.9% | 99.7% | 1162G>C |
| 2 | human | 8846 | ALKBH1 | alkB homolog 1, histone H2A... | XR_001750582.1 | 42.5% | (many diffs) | |
| 3 | mouse | 211064 | Alkbh1 | alkB homolog 1, histone H2A... | NM_001102565.1 | 84.5% | 82.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1233
- ORF length:
- 1167
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaagatggca gcggccgtgg gctctgtggc gactctggcg actgagcccg 121 gggaggacgc ctttcggaaa cttttccgct tctaccgtca gagccggccc gggaccgcag 181 acctggaagg ggtcatcgac ttctcggcgg cccacgcagc ccgtggcaag ggtcctggtg 241 cccaaaaggt gatcaaatct cagctaaatg tgtcttctgt cagtgagcag aatgcatata 301 gagcaggtct tcagcccgtc agcaagtggc aagcctatgg actcaaaggc tatcctgggt 361 ttatttttat cccaaacccc ttcctcccag gttaccagtg gcactgggtg aaacagtgcc 421 ttaagttata ttcccagaaa cctaatgtat gtaacctgga caaacacatg tctaaagaag 481 agacccaaga tctgtgggaa cagagcaaag agttcctgag gtataaagaa gcgactaaac 541 ggagaccccg aagtttactg gagaaactgc gttgggtgac cgtaggctac cattataact 601 gggacagtaa gaaatactca gcagatcatt acacaccttt cccttctgac ctgggtttcc 661 tctcagagca agtagccgct gcctgtggat ttgaggattt ccgagctgaa gcagggatcc 721 tgaattacta ccgcctggac tccacactgg gaatccacgt agacagatct gagctagatc 781 actccaaacc cttgctgtca ttcagctttg gacagtccgc catctttctc ctgggtggtc 841 ttcaaaggga tgaggccccc acggccatgt ttatgcacag tggtgacatc atgataatgt 901 cgggtttcag ccgcctcttg aaccacgcag tccctcgtgt ccttccaaat ccagaagggg 961 aaggcctgcc tcactgccta gaggcacctc tccctgctgt cctcccgaga gattcaatgg 1021 tagagccttg ttctatggag gactggcagg tgtgtgccag ctacttgaag accgctcgtg 1081 ttaacatgac tgtccgacag gtcctggcca cagaccagaa tttccctcta gaacccatcg 1141 aggatgaaaa aagagacatc agtacagaag gtttcTGCCA TCTGGATGAC CAGAATAGCG 1201 AAGTAAAACG GGCCAGGATA AACCCTCACA GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1261 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1321 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 1381 AGCACTATGC GCTCTGTGTC GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1441 ttgtgaaaga tt