Transcript: Human NM_006020.3

Homo sapiens alkB homolog 1, histone H2A dioxygenase (ALKBH1), mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
ALKBH1 (8846)
Length:
2597
CDS:
20..1189

Additional Resources:

NCBI RefSeq record:
NM_006020.3
NBCI Gene record:
ALKBH1 (8846)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006020.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000235017 GACCGTAGGCTACCATTATAA pLKO_005 532 CDS 100% 15.000 21.000 N ALKBH1 n/a
2 TRCN0000235019 CTTGAAGACCGCTCGTGTTAA pLKO_005 1018 CDS 100% 13.200 18.480 N ALKBH1 n/a
3 TRCN0000146838 CCACTCCATCTGGTAATTTAT pLKO.1 1571 3UTR 100% 15.000 10.500 N ALKBH1 n/a
4 TRCN0000235021 CTCCACTCCATCTGGTAATTT pLKO_005 1569 3UTR 100% 15.000 10.500 N ALKBH1 n/a
5 TRCN0000235018 GAAATACTCAGCAGATCATTA pLKO_005 565 CDS 100% 13.200 9.240 N ALKBH1 n/a
6 TRCN0000239212 GAAATACTCAGCAGATCATTA pLKO_005 565 CDS 100% 13.200 9.240 N Alkbh1 n/a
7 TRCN0000235020 TGACCAGAATAGCGAAGTAAA pLKO_005 1141 CDS 100% 13.200 9.240 N ALKBH1 n/a
8 TRCN0000147996 GAGGTATAAAGAAGCGACTAA pLKO.1 472 CDS 100% 4.950 3.465 N ALKBH1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006020.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07312 pDONR223 100% 99.9% 99.7% None 1162G>C n/a
2 ccsbBroad304_07312 pLX_304 0% 99.9% 99.7% V5 1162G>C n/a
3 TRCN0000472177 CGAGCACTATGCGCTCTGTGTCGC pLX_317 5.7% 99.9% 99.7% V5 1162G>C n/a
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