Construct: ORF TRCN0000472178
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017664.1_s317c1
- Derived from:
- ccsbBroadEn_03099
- DNA Barcode:
- TGTCGACCGAAGAGTGCGGCATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRA2A (29896)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472178
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29896 | TRA2A | transformer 2 alpha homolog | NM_013293.5 | 100% | 100% | |
| 2 | human | 29896 | TRA2A | transformer 2 alpha homolog | NM_001362759.2 | 77.6% | 77.6% | 335_336ins189 |
| 3 | human | 29896 | TRA2A | transformer 2 alpha homolog | NM_001282757.1 | 64.1% | 64.1% | 0_1ins303 |
| 4 | human | 29896 | TRA2A | transformer 2 alpha homolog | NM_001282758.1 | 64.1% | 64.1% | 0_1ins303 |
| 5 | human | 29896 | TRA2A | transformer 2 alpha homolog | NM_001362760.2 | 64.1% | 64.1% | 0_1ins303 |
| 6 | human | 29896 | TRA2A | transformer 2 alpha homolog | NM_001362761.2 | 64.1% | 64.1% | 0_1ins303 |
| 7 | human | 29896 | TRA2A | transformer 2 alpha homolog | XM_017012090.1 | 64.1% | 64.1% | 0_1ins303 |
| 8 | human | 29896 | TRA2A | transformer 2 alpha homolog | NM_001282759.1 | 63.8% | 63.8% | 0_1ins303;465_466insAGA |
| 9 | mouse | 101214 | Tra2a | transformer 2 alpha homolog... | NM_198102.2 | 88.9% | 98.5% | (many diffs) |
| 10 | mouse | 101214 | Tra2a | transformer 2 alpha homolog... | NM_001347209.1 | 88.3% | 97.8% | (many diffs) |
| 11 | mouse | 101214 | Tra2a | transformer 2 alpha homolog... | XM_006505240.1 | 81.2% | 90% | (many diffs) |
| 12 | mouse | 101214 | Tra2a | transformer 2 alpha homolog... | XM_006505241.1 | 81% | 89.7% | (many diffs) |
| 13 | mouse | 101214 | Tra2a | transformer 2 alpha homolog... | XM_006505242.1 | 80.7% | 89.3% | (many diffs) |
| 14 | mouse | 101214 | Tra2a | transformer 2 alpha homolog... | XM_006505243.1 | 80.3% | 89% | (many diffs) |
| 15 | mouse | 101214 | Tra2a | transformer 2 alpha homolog... | XM_006505245.1 | 56.9% | 63.7% | (many diffs) |
| 16 | mouse | 101214 | Tra2a | transformer 2 alpha homolog... | XM_011241102.1 | 56.9% | 63.7% | (many diffs) |
| 17 | mouse | 101214 | Tra2a | transformer 2 alpha homolog... | XM_017321288.1 | 56.6% | 63.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 912
- ORF length:
- 846
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgatgtggag gaaaacaact tcgagggcag agagtctcgc tctcagtcaa 121 aatctccaac gggaactcct gctcgtgtaa aatcggagag caggtcagga tctcgtagtc 181 catcaagggt ttccaaacac tctgaatccc attctcgatc aagatcaaaa tccaggtcga 241 ggtcaaggag acattctcat agacgttaca ctcgatccag atcccactct cactctcata 301 ggagacgatc tcgaagtaga tcatatacac cagaataccg gcggcgaagg agccgaagcc 361 attctccaat gtctaaccgg agaagacata ctggcagcag ggcaaatcca gatcccaaca 421 cttgccttgg agtgtttggc ctcagtttgt acacaacaga gagggatctt cgtgaagtat 481 tttctcgata tggaccattg agtggtgtca atgtggttta tgatcagcga actgggcgat 541 ctcgaggatt tgcttttgtg tattttgaga gaatagatga ctcaaaggag gctatggaaa 601 gggcaaatgg aatggagctg gatggtagaa gaattcgggt ggattattct ataaccaaga 661 gagcgcacac accaacacca ggcatctaca tgggcagacc aactcatagt ggtgggggtg 721 gtggaggagg cggcggcggt ggaggtggag gtggtggcag acgtcgagat tcttactatg 781 atagaggata tgatcgtggg tatgacagat atgaagacta tgattaccga tacagaagac 841 gatcaccttc tccttaTTAT AGTCGATATA GATCACGATC AAGATCTCGT TCCTACAGCC 901 CAAGACGCTA TTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 961 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1021 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGT CGACCGAAGA GTGCGGCATT 1081 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t