Construct: ORF TRCN0000472180
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012313.1_s317c1
- Derived from:
- ccsbBroadEn_02893
- DNA Barcode:
- CACCTTAGATAGAAAAGAGCAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UNC50 (25972)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472180
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25972 | UNC50 | unc-50 inner nuclear membra... | NM_014044.6 | 100% | 100% | |
| 2 | human | 25972 | UNC50 | unc-50 inner nuclear membra... | NM_001330353.2 | 95.2% | 76.9% | 643_664del;783_784insTAAGTACAGAGTGAAA |
| 3 | human | 25972 | UNC50 | unc-50 inner nuclear membra... | NM_001330354.1 | 93.8% | 93.8% | 1_51del |
| 4 | human | 25972 | UNC50 | unc-50 inner nuclear membra... | XM_006712403.3 | 89.5% | 72.6% | 1_51del;694_715del;834_835insTAAGTACAGAGTGAAA |
| 5 | mouse | 67387 | Unc50 | unc-50 homolog (C. elegans) | NM_026123.3 | 86.7% | 95.7% | (many diffs) |
| 6 | mouse | 67387 | Unc50 | unc-50 homolog (C. elegans) | XM_006496220.3 | 86.7% | 95.7% | (many diffs) |
| 7 | mouse | 67387 | Unc50 | unc-50 homolog (C. elegans) | XM_006496221.3 | 86.7% | 95.7% | (many diffs) |
| 8 | mouse | 67387 | Unc50 | unc-50 homolog (C. elegans) | XM_006496222.3 | 86.7% | 95.7% | (many diffs) |
| 9 | mouse | 67387 | Unc50 | unc-50 homolog (C. elegans) | XM_006496223.3 | 86.7% | 95.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 843
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt accgagtact tcagtgaatt ccttagtgca ggggaacgga gtcttgaatt 121 ccagggatgc ggcaagacac acagccggag cgaaacgcta caaatatctg agaaggcttt 181 tccgctttcg gcaaatggac tttgaatttg ctgcctggca gatgctctac ctgttcacat 241 ccccacagag agtttacaga aattttcatt atcgaaaaca gacgaaggac cagtgggcca 301 gagatgaccc tgctttcttg gtcctgttaa gtatctggct ctgtgtgtcc actataggat 361 ttGGCTTTGT GCTGGACATG GGATTCTTTG AGACAATAAA GCTTCTCCTT TGGGTTGTAC 421 TCATAGATTG TGTAGGCGTT GGTCTTCTGA TAGCAACTTT AATGTGGTTC ATCTCTAACA 481 AGTATTTAGT GAAACGACAG AGCAGAGACT ATGATGTGGA ATGGGGCTAT GCTTTTGATG 541 TGCATCTCAA TGCTTTTTAT CCACTCCTGG TCATTTTGCA TTTTATCCAG CTTTTTTTCA 601 TCAACCATGT TATCCTGACA GACACATTTA TTGGATATTT AGTTGGAAAT ACCTTATGGT 661 TGGTTGCAGT TGGCTATTAT ATCTATGTAA CTTTCCTGGG ATACAGTGCA TTGCCATTTT 721 TGAAAAATAC AGTAATTCTT CTGTATCCAT TTGCACCTCT GATTCTGCTC TACGGGCTTT 781 CCCTGGCACT GGGATGGAAC TTCACCCATA CTCTCTGTTC TTTCTATAAG TACAGAGTGA 841 AATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 901 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 961 GGCTTTATAT ATCTTGTGGA AAGGACGACA CCTTAGATAG AAAAGAGCAA CTACGCGTTA 1021 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt