Construct: ORF TRCN0000472180

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012313.1_s317c1
Derived from:
ccsbBroadEn_02893
DNA Barcode:
CACCTTAGATAGAAAAGAGCAACT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UNC50 (25972)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472180
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25972 UNC50 unc-50 inner nuclear membra... NM_014044.6 100% 100%
2 human 25972 UNC50 unc-50 inner nuclear membra... NM_001330353.2 95.2% 76.9% 643_664del;783_784insTAAGTACAGAGTGAAA
3 human 25972 UNC50 unc-50 inner nuclear membra... NM_001330354.1 93.8% 93.8% 1_51del
4 human 25972 UNC50 unc-50 inner nuclear membra... XM_006712403.3 89.5% 72.6% 1_51del;694_715del;834_835insTAAGTACAGAGTGAAA
5 mouse 67387 Unc50 unc-50 homolog (C. elegans) NM_026123.3 86.7% 95.7% (many diffs)
6 mouse 67387 Unc50 unc-50 homolog (C. elegans) XM_006496220.3 86.7% 95.7% (many diffs)
7 mouse 67387 Unc50 unc-50 homolog (C. elegans) XM_006496221.3 86.7% 95.7% (many diffs)
8 mouse 67387 Unc50 unc-50 homolog (C. elegans) XM_006496222.3 86.7% 95.7% (many diffs)
9 mouse 67387 Unc50 unc-50 homolog (C. elegans) XM_006496223.3 86.7% 95.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
843
ORF length:
777
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt accgagtact tcagtgaatt ccttagtgca ggggaacgga gtcttgaatt
121ccagggatgc ggcaagacac acagccggag cgaaacgcta caaatatctg agaaggcttt
181tccgctttcg gcaaatggac tttgaatttg ctgcctggca gatgctctac ctgttcacat
241ccccacagag agtttacaga aattttcatt atcgaaaaca gacgaaggac cagtgggcca
301gagatgaccc tgctttcttg gtcctgttaa gtatctggct ctgtgtgtcc actataggat
361ttGGCTTTGT GCTGGACATG GGATTCTTTG AGACAATAAA GCTTCTCCTT TGGGTTGTAC
421TCATAGATTG TGTAGGCGTT GGTCTTCTGA TAGCAACTTT AATGTGGTTC ATCTCTAACA
481AGTATTTAGT GAAACGACAG AGCAGAGACT ATGATGTGGA ATGGGGCTAT GCTTTTGATG
541TGCATCTCAA TGCTTTTTAT CCACTCCTGG TCATTTTGCA TTTTATCCAG CTTTTTTTCA
601TCAACCATGT TATCCTGACA GACACATTTA TTGGATATTT AGTTGGAAAT ACCTTATGGT
661TGGTTGCAGT TGGCTATTAT ATCTATGTAA CTTTCCTGGG ATACAGTGCA TTGCCATTTT
721TGAAAAATAC AGTAATTCTT CTGTATCCAT TTGCACCTCT GATTCTGCTC TACGGGCTTT
781CCCTGGCACT GGGATGGAAC TTCACCCATA CTCTCTGTTC TTTCTATAAG TACAGAGTGA
841AATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
901TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
961GGCTTTATAT ATCTTGTGGA AAGGACGACA CCTTAGATAG AAAAGAGCAA CTACGCGTTA
1021AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)