Construct: ORF TRCN0000472183
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016155.1_s317c1
- Derived from:
- ccsbBroadEn_08524
- DNA Barcode:
- TACGCCGTAATGAACCATCCGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GIMAP5 (55340)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472183
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55340 | GIMAP5 | GTPase, IMAP family member 5 | NM_018384.4 | 99.8% | 99.6% | 913C>G |
| 2 | human | 100527949 | GIMAP1-GIMAP5 | GIMAP1-GIMAP5 readthrough | NM_001303630.1 | 80% | 79.8% | 1_228del;1141C>G |
| 3 | human | 100527949 | GIMAP1-GIMAP5 | GIMAP1-GIMAP5 readthrough | NM_001199577.1 | 60% | 59.8% | 1_612del;1525C>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 987
- ORF length:
- 921
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aggattccag aggggcaaat atggaactat ggctgaaggt agatcagaag 121 ataacttgtc tgcaacacca ccggcattga ggattatcct agtgggcaaa acaggctgcg 181 ggaaaagtgc cacagggaac agcatccttg gccagcccgt gtttgagtcc aagctgaggg 241 cccagtcagt gaccaggacg tgccaggtga aaacaggaac atggaacggg aggaaagtcc 301 tggtggttga cacgccctcc atctttgagt cacaggccga tacccaagag ctgtacaaga 361 acatcgggga ctgctacctg ctctctgccc cggggcccca cgtcctgctt ctggtgatcc 421 agctggggcg tttcactgct caggacacag tggccatcag gaaggtgaaa gaggtctttg 481 ggacaggggc catgagacat gtggtcatcc tcttcaccca caaagaggac ttagggggCC 541 AGGCCCTGGA TGACTATGTA GCAAACACGG ACAACTGCAG CCTGAAAGAC CTGGTGCGGG 601 AGTGTGAGAG AAGGTACTGT GCCTTCAACA ACTGGGGCTC TGTGGAGGAG CAGAGGCAGC 661 AGCAGGCAGA GCTCCTGGCT GTGATTGAGA GGCTGGGGAG GGAGCGAGAG GGCTCCTTCC 721 ACAGCAATGA CCTCTTCTTG GATGCCCAGC TGCTCCAAAG AACTGGAGCT GGGGCCTGCC 781 AGGAAGACTA CAGGCAGTAC CAGGCCAAAG TGGAATGGCA GGTGGAGAAG CACAAGCAAG 841 AGCTGAGGGA GAACGAGAGT AACTGGGCAT ACAAGGCGCT CCTCAGAGTC AAACACTTGA 901 TGCTTTTGCA TTATGAGATT TTTGTTTTTC TATTGTTGTG CAGCATACTT TTTTTCATTA 961 TTTTTCTGTT CATCTTTGAT TACATTTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1021 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1081 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATACGCCGT 1141 AATGAACCAT CCGAGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1201 aagatt