Construct: ORF TRCN0000472187
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011602.1_s317c1
- Derived from:
- ccsbBroadEn_03011
- DNA Barcode:
- AATCTTGACCTTACTTGCCGGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHMP2A (27243)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472187
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27243 | CHMP2A | charged multivesicular body... | NM_014453.4 | 100% | 100% | |
| 2 | human | 27243 | CHMP2A | charged multivesicular body... | NM_198426.3 | 100% | 100% | |
| 3 | human | 27243 | CHMP2A | charged multivesicular body... | XM_005258746.3 | 83.7% | 83.3% | 542_670del |
| 4 | human | 27243 | CHMP2A | charged multivesicular body... | XM_005258747.3 | 83.7% | 83.3% | 542_670del |
| 5 | mouse | 68953 | Chmp2a | charged multivesicular body... | NM_026885.3 | 90.6% | 99.5% | (many diffs) |
| 6 | mouse | 68953 | Chmp2a | charged multivesicular body... | XM_006540345.3 | 90.6% | 99.5% | (many diffs) |
| 7 | mouse | 68953 | Chmp2a | charged multivesicular body... | XM_006540344.1 | 80.5% | 88.4% | (many diffs) |
| 8 | mouse | 68953 | Chmp2a | charged multivesicular body... | XM_011250690.1 | 80.5% | 88.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 732
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cctattgttc gggcgccgga agacgccaga ggagctactg cggcagaacc 121 agagggccct gaaccgtgcc atgcgggagc tggaccgcga gcgacagaaa ctagagaccc 181 aggagaagaa aatcattgca gacattaaga agatggccaa gcaaggccag atggatgctg 241 ttcgcatcat ggcaaaagac ttggtgcgca cccGGCGCTA TGTGCGCAAG TTTGTATTGA 301 TGCGGGCCAA CATCCAGGCT GTGTCCCTCA AGATCCAGAC ACTCAAGTCC AACAACTCGA 361 TGGCACAAGC CATGAAGGGT GTCACCAAGG CCATGGGCAC CATGAACAGA CAGCTGAAGT 421 TGCCCCAGAT CCAGAAGATC ATGATGGAGT TTGAGCGGCA GGCAGAGATC ATGGATATGA 481 AGGAGGAGAT GATGAATGAT GCCATTGATG ATGCCATGGG TGATGAGGAA GATGAAGAGG 541 AGAGTGATGC TGTGGTGTCC CAGGTTCTGG ATGAGCTGGG ACTTAGCCTA ACAGATGAGC 601 TGTCGAACCT CCCCTCAACT GGGGGCTCGC TTAGTGTGGC TGCTGGTGGG AAAAAAGCAG 661 AGGCCGCAGC CTCAGCCCTA GCTGATGCTG ATGCAGACCT GGAGGAACGG CTTAAGAACC 721 TGCGGAGGGA CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 781 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG CCCGTAACTT GAAAGTATTT 841 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAT CTTGACCTTA CTTGCCGGGT 901 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t