Transcript: Human XM_005258746.3

PREDICTED: Homo sapiens charged multivesicular body protein 2A (CHMP2A), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CHMP2A (27243)
Length:
1023
CDS:
143..940

Additional Resources:

NCBI RefSeq record:
XM_005258746.3
NBCI Gene record:
CHMP2A (27243)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005258746.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000140813 GCAGGCAGAGATCATGGATAT pLKO.1 535 CDS 100% 10.800 7.560 N CHMP2A n/a
2 TRCN0000143825 GCTATGTGCGCAAGTTTGTAT pLKO.1 354 CDS 100% 5.625 3.938 N CHMP2A n/a
3 TRCN0000144104 CAGAAGATCATGATGGAGTTT pLKO.1 509 CDS 100% 4.950 3.465 N CHMP2A n/a
4 TRCN0000143644 CATGAACAGACAGCTGAAGTT pLKO.1 478 CDS 100% 4.950 3.465 N CHMP2A n/a
5 TRCN0000140662 CCTCAAGATCCAGACACTCAA pLKO.1 403 CDS 100% 4.950 3.465 N CHMP2A n/a
6 TRCN0000145250 GAAGATCATGATGGAGTTTGA pLKO.1 511 CDS 100% 4.950 3.465 N CHMP2A n/a
7 TRCN0000138967 CACCATGAACAGACAGCTGAA pLKO.1 475 CDS 100% 4.050 2.835 N CHMP2A n/a
8 TRCN0000122479 CATGGATATGAAGGAGGAGAT pLKO.1 547 CDS 100% 4.050 2.835 N CHMP2A n/a
9 TRCN0000122300 CCAGAAGATCATGATGGAGTT pLKO.1 508 CDS 100% 4.050 2.835 N CHMP2A n/a
10 TRCN0000139572 CATTGATGATGCCATGGGTGA pLKO.1 580 CDS 100% 2.160 1.512 N CHMP2A n/a
11 TRCN0000122604 GCCAAGCAAGGCCAGATGGAT pLKO.1 293 CDS 100% 1.000 0.700 N CHMP2A n/a
12 TRCN0000138966 CTAGAGACCCAGGAGAAGAAA pLKO.1 248 CDS 100% 5.625 3.375 N CHMP2A n/a
13 TRCN0000139373 CCAGACACTCAAGTCCAACAA pLKO.1 412 CDS 100% 4.950 2.970 N CHMP2A n/a
14 TRCN0000142935 CCAGATCCAGAAGATCATGAT pLKO.1 502 CDS 100% 4.950 2.970 N CHMP2A n/a
15 TRCN0000143522 GAAGATGAAGAGGAGAGTGAT pLKO.1 605 CDS 100% 4.950 2.970 N CHMP2A n/a
16 TRCN0000140257 GAAACTAGAGACCCAGGAGAA pLKO.1 244 CDS 100% 4.050 2.430 N CHMP2A n/a
17 TRCN0000200397 GAAGAGGAGAGTGATGCTGTT pLKO.1 611 CDS 100% 4.050 2.430 N Chmp2a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005258746.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03011 pDONR223 100% 83.7% 83.3% None 542_670del n/a
2 ccsbBroad304_03011 pLX_304 0% 83.7% 83.3% V5 542_670del n/a
3 TRCN0000472187 AATCTTGACCTTACTTGCCGGGTG pLX_317 68.3% 83.7% 83.3% V5 542_670del n/a
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