Construct: ORF TRCN0000472200
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010735.1_s317c1
- Derived from:
- ccsbBroadEn_12339
- DNA Barcode:
- AATTGTACGTGGGCCGATGGTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMAGP (57228)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472200
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57228 | SMAGP | small cell adhesion glycopr... | NM_001031628.2 | 25% | 3% | 1_205del;291_292ins52 |
| 2 | human | 57228 | SMAGP | small cell adhesion glycopr... | NM_001033873.1 | 25% | 3% | 1_205del;291_292ins52 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 320
- ORF end:
- 458
- ORF length:
- 138
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA TGAACCTACA GAAGGTGAGC CCAGTGCCAT CGTCCAGATG 361 GAGAGTGACT TGGCCAAGGG CAGCGAGAAA GAGGAATATT TCATCTAATG ACTCCCAGGC 421 CCCAAGGAGC TTATTCCTGG CTCCATCGCT AACACGTTGC CCAACTTTCT TGTACAAAGT 481 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 541 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 601 CGAAATTGTA CGTGGGCCGA TGGTCACACG CGTTAAGTCg acaatcaacc tctggattac 661 aaaatttgtg aaagatt