Construct: ORF TRCN0000472202
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018957.3_s317c1
- Derived from:
- ccsbBroadEn_14956
- DNA Barcode:
- GATCCTCATTAAATCGTAGATTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LRPPRC (10128)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472202
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10128 | LRPPRC | leucine rich pentatricopept... | NM_133259.4 | 99.5% | 15.3% | (many diffs) |
| 2 | human | 10128 | LRPPRC | leucine rich pentatricopept... | XM_011532473.1 | 97.7% | 15.6% | (many diffs) |
| 3 | human | 10128 | LRPPRC | leucine rich pentatricopept... | XM_006711915.2 | 96.5% | 12.4% | (many diffs) |
| 4 | human | 10128 | LRPPRC | leucine rich pentatricopept... | XM_017003117.1 | 94.7% | 12.6% | (many diffs) |
| 5 | human | 10128 | LRPPRC | leucine rich pentatricopept... | XM_006711916.3 | 75% | 20.3% | (many diffs) |
| 6 | human | 10128 | LRPPRC | leucine rich pentatricopept... | XR_002958896.1 | 61.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 744
- ORF length:
- 675
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagccctg ctgagatccg cgcgttggtt gctgcgtgcc ggggcggccc 121 cgcgcctccc gctctccctg cgcctcctcc ctggcggccc gggccggctg catgccgcct 181 cctatctgcc cgccgctcgc gccgggcccg tggccggagg actactgagc ccagccaggc 241 tgtatgccat tgctgccaaa gaaaaagata ttcaagagga gtccactttt tcttctagga 301 agatttccaa tcaatttgat tgggctctaa tgagactaga tctttctgtt cgaagaactg 361 gccgcattcc aaagaagctt ctacaaaaag tttttaatga tacctgccgc tcaggtggcc 421 taggtggtag tcatgccttg cttctactac gtagttgtgg ttctctcttg cctgaactaa 481 agcttgaaga gagaacagaa tttgctcata ggatatggga cacacttcag aaattaggtg 541 ctgtgtatga tgtgagtcac tataatgctt tacttaaagt ctatcttcaa aatgaatata 601 aattctcacc aactgatttc ctggcaaaaa tggaggaagc aaacattcaa ccaaatcgag 661 tgacatacca gagattgatt gcttcttatt gtaatgtagg agatattgaa aggtgccagc 721 aagattcttg gatttatgaa aactaaagga tctcccagtt acagaggcag tattcagtgc 781 ccttgtgaca gggcatgcca agagctggtg ataatggaga atgcagaaaa acagtgatga 841 gagatgccgg aattgagcct ggtccagaca catacctcgc attattgaat gcatatgctg 901 agaagggcga cattgaccat gttaagcaga ctctggagaa ggtggagaag tccgagcttc 961 accttatgga ccgtgattta ctgcaaatta tttttagctt cagtaaagct gggtatcctc 1021 agtatgtctc agaaattttg gaaaaagtta catgtgaaag aagatatatt ccagatgcaa 1081 tgaacctcat tttactttta gtcactgaaa aattggaaga tgtagcgttg caaattttac 1141 tagcatgccc cgtatcaaag gaagatggcc caagtgtctt tggcagtttc tttttacaac 1201 actgtgtgac tatgaatacg cctgtggaga agctaacaga ctactgtaag aagttaaagg 1261 aagtccagat gcactccttt cctctgcagt tcaccctcca ttgtgcttta ctcgccaata 1321 aaactgattt ggcaaaagcc ttaatgaagg ctgtgaagga ggaaggtttt cctatcagac 1381 ctcactattt ctggccattg ctagttggac gtcggaagga aaaaaatgtt caaggtataa 1441 ttgaaatcct caaaggaatg caagaattgg gagtacatcc tgatcaggaa acatatacag 1501 attatgtgat tccatgcttt gatagtgtaa actcagcacg agccattttg caggaaaatg 1561 gatgtctgtc tgatagtgat atgttttctc aagctggatt gagaagtgaa gcagcaaatg 1621 ggaacttaga ctttgtatta tcatttttga aatcaaatac attgcccatc tcgctgcagt 1681 ctataagaag tagcctactg ctaggcttca ggaggtctat gaatataaat ctttggagcg 1741 agataacaga attgttgtac aaggatggac gttattgcca ggagcctcga ggaccgacgg 1801 aagctgttgg ctattttctt tataacttga ttgacagcat gagtgactca gaagtacagg 1861 ccaaggagga gcatttgaga caatacttcc atcagctgga gaagatgaat gtaaaaattc 1921 ctgaaaatat ctacagaggc attcgtaatc tcctggaaag ctaccatgtt cctgaattga 1981 ttaaggatgc tcacttgttg gttgagagta agaatttaga ctttcaaaaa actgtgcaac 2041 ttacatcatc tgaattggag tccacacttg aaacactaaa agctgaaaat caacctataa 2101 gagatgtcct aaagcaactc atattagtgc tttgttcaga agagaatatg caaaaagccc 2161 ttgaattgaa agcaaaatat gaatccgaca tggttactgg tggctatgca gctttaataa 2221 atttatgctg tcgacatgat aaagtagaag atgccttgaa cttgaaagaa gaatttgacc 2281 gcttagattc atctgctgtc cttgacaccg gcaagtatgt aggccttgta agagtattgg 2341 caaagcatgg caagctccaa gatgctatta acattctgaa ggagatgaaa gagaaggatg 2401 ttcttatcaa agatacaaca gccttgtcct ttttccacat gctaaatggc gcagctttaa 2461 gaggtgaaat tgaaacagta aaacagttgc atgaagccat cgtgactcta gggttagcag 2521 aaccatccac caacataagt ttcccattgg tcactgtaca cttggaaaag ggcgacctat 2581 ctactgctct tgaggtcgcc attgactgct atgaaaagta taaagtatta ccaaggattc 2641 atgatgtctt gtgtaaactg gtagagaaag gcgagactga tctaattcag aaagcaatgg 2701 actttgtgag ccaagaacaa ggtgaaatgg tgatgctcta tgatctcttc tttgccttcc 2761 tacaaacagg aaattacaaa gaggccaaga agatcattga gactccaggg attagagctc 2821 gatctgcaag gcttcagtgg ttttgtgaca gatgtgttgc aaataatcag gttgaaactc 2881 tggaaaaatt agtggagctg acacagaagc tatttgaatg tgatagagac cagatgtact 2941 acaatctgct aaaactgtat aaaataaacg gtgactggca aagagctgat gcagtctgga 3001 ataaaatcca agaagaaaat gttattcctc gtgaaaagac attaagatta ttagcagaaa 3061 tccttagaga gggtaaccag gaagttccgt ttgacgtacc tgagttgtgg tatgaagatg 3121 aaaaacattc cctgaattct tcgtcagcct caaccacaga acctgatttc cagaaagata 3181 tattgattgc ctgccgattg aaccaaaaaa aaggggcata tgatattttc ctgaatgcaa 3241 aagagcaaaa cattgtgttt aatgctgaaa cctacagcaa tctcattaaa ttactgatgt 3301 cagaagatta ttttacacaa gcaatggaag tgaaagcatt cgcggagacc cacatcaagg 3361 gcttcacact gaacgatgct gccaacagcc gcctcatcat aacgcaagtt aggcgggatt 3421 atttgaaaga ggctgtgaca acactgaaaa cagtattgga tcagcagcag acccgttcta 3481 ggttagccag tgacccgtgg tcatccaggc attgggccat gaaagggtga tgttgaaaac 3541 atagaagtag ttcagaagat gttaaatgga ctcgaagact ccattggact tcaaaaatgg 3601 ttttcatcaa taacattgct ttggctcaaa taaagaataa taacatagat gccgcaatag 3661 aaaacattga aaatatgctt acttcagaga ataaagtcat tgaaccccaa tacttcggct 3721 tggcatactt attcagaaaa gtaatagagg agcagttgga accagcagtt gaaaagataa 3781 gcatcatggc ggagagattg gccaatcagt ttgcaattta taaacctgtc actgattttt 3841 tccTTCAACT TGTGGATGCA GGCAAGGTGG ATGATGCCAG AGCTCTCCTA CAGAGATGTG 3901 GTGCAATTGC TGAACAAACC CCGATTTTGT TGTTGTTCCT CCTTAGGAAT TCTAGGAAAC 3961 AAGGAAAGGC ATCAACTGTG AAATCTGTGT TAGAATTGAT TCCTGAATTA AATGAAAAGG 4021 AAGAAGCATA CAATTCCCTC ATGAAAAGCT ATGTCTCAGA GAAAGATGTC ACATCTGCTA 4081 AAGCACTGTA TGAACATTTG ACTGCAAAGA ATACAAAATT GGATGATCTG TTTCTAAAGC 4141 GTTACGCATC TTTGCTGAAG TATGCTGGAG AGCCTGTCCC TTTCATTGAA CCCCCTGAAA 4201 GCTTTGAATT TTATGCACAG CAGCTAAGAA AATTGAGGGA AAACTCTTCT TTGCCAACTT 4261 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 4321 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 4381 CTTGTGGAAA GGACGAGATC CTCATTAAAT CGTAGATTCG ACGCGTTAAG TCgacaatca 4441 acctctggat tacaaaattt gtgaaagatt