Construct: ORF TRCN0000472214
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002237.1_s317c1
- Derived from:
- ccsbBroadEn_04124
- DNA Barcode:
- TATATTGTAAGCCACCTATCACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IQCA1 (79781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472214
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79781 | IQCA1 | IQ motif containing with AA... | NM_024726.5 | 100% | 100% | |
| 2 | human | 79781 | IQCA1 | IQ motif containing with AA... | XM_011511865.3 | 99.8% | 99.8% | 1411_1413delAAG |
| 3 | human | 79781 | IQCA1 | IQ motif containing with AA... | XM_011511864.2 | 99% | 98.7% | 4A>T;7_27del;31_32delAGinsGC |
| 4 | human | 79781 | IQCA1 | IQ motif containing with AA... | NM_001270585.1 | 98.9% | 98.6% | (many diffs) |
| 5 | human | 79781 | IQCA1 | IQ motif containing with AA... | NM_001270584.1 | 95% | 95% | 1009_1010ins123 |
| 6 | human | 79781 | IQCA1 | IQ motif containing with AA... | XM_017004960.1 | 94% | 93.8% | (many diffs) |
| 7 | human | 79781 | IQCA1 | IQ motif containing with AA... | XM_011511866.1 | 93.9% | 93.7% | (many diffs) |
| 8 | human | 79781 | IQCA1 | IQ motif containing with AA... | NR_073043.2 | 75.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2532
- ORF length:
- 2466
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gaacgcaatg tataataaga tgtggcatca gacccaagaa gccctcggtg 121 ctttactcga taaagagcct cagaagatga ttgaaccaca aagaaatcag gttttcatct 181 ttcaaacatt agccaccttc tacgtaaagt atgtgcagat ctttagaaac ctagagaatg 241 tctacgacca gttcgtccac ccccagaaac gaatactgat caggaaagtc ctggacgggg 301 tgatgggccg catcctggag ctgaagaacg agatggtgga gctggaactc acggagttcc 361 attatttcga tgatatcctg caggatctca agctggcccc ccaacaatta gatattccca 421 tacccaagta ttttttgaag gagaagttgg aagtaataaa aggaagagag aaaattcttg 481 ctcaaatatt agcggacagc ggaatagata catctgacat gaaataccct gtaaagagta 541 tccctttcga tgaggctgtt aaattaatcc agattgctga gagggcacgg caaggtcgcc 601 taagggcttt attcatgaag caaatctatc tgcaagaata tagagcaaag caatccaaga 661 tgcttggcaa gaaagtgaca gatacctggg ctgctgcact ccgcattcag aaggtttggc 721 gacgtttcca tcaacgtaag gaaactgaaa aactgagaga agaggagatg atcttcctgg 781 gtatgaatcc acctcctctc tttaatgaag tcagtgctac agtaatccag gctgaaaagg 841 tggaccgcct gcggaatgag gtgcagataa agcatgaaga ggactacagg gaagccctgg 901 ttaccatcaa gaatgaccta aagttgatag aaggcgtgga tatcaaggag aaccttcaag 961 accagatccg gcattggttc atcgaatgca gaaatttaac cgggacattt cctgactacc 1021 ctgacgttga agaaggaggg tcagctatta ttttttctga caagaccata caacaggtta 1081 ttgaggatat catagcaaac caagaggaag aagaaaaaaa caaaaagaag aagaagaaaa 1141 aggaaaaaca acccaagaaa gccaaaaaac aaaagaaagg aacaaaggag aaaaataagg 1201 aagaagacga aaaatggaaa atgtcaccaa gtctttttct tcctgcaatg aaggaaggat 1261 gtaacgcata caaagaaatc tggatgaaaa aagatgagtc ttggaatttc tctcaggact 1321 atgatccaga actgatcaaa gaggagaaac gaaaagaatt acagtcagag atcaggatac 1381 aggttgatga gttgatgaga caggaactta aaaacttaaa gctagctgtg gacagagaaa 1441 gggagcgccc agtgaaagca ggaaagaaaa aggacaagaa aggaaagaaa ggcaaaaaga 1501 aagagaagaa ggcaaagaag gataaagatc tgacagctga caggaccatc gagtctctgt 1561 ataaggaact ggtggaagaa ggattactga tccaggctct gaaagtcaac ctctctgatt 1621 acattggtga gtacagctac ctggggacta ctcttcgcca ggtgtccata gaacccatgc 1681 cctccctcct ggatgtcaga cagctcatca cattgtacgg aatctggcca ttaggttctg 1741 cagcagtgca tgagaaagct cctttggtga aatcgctact gttagccggg ccgtctgggg 1801 tagggaagaa aatgctggtc catgccatct gcaccgaaac gggagccaac ctcttcaact 1861 tgtcctcctc taacattgct gggaaatacc ctggcaaaaa tggcctccaa atgatgctgc 1921 atgcagtctt caaggtggct cgacagctcc agccctccgt ggtgtggatt gaagacacag 1981 aaaaaacctt ctacaaaaaa gttcccaacg cagaaaagat gaatgaacct aaacgcctga 2041 aaaaacacct tccccaaatc ctgaaactcc tgaaaccaga tgaccggatt ctgattgtgg 2101 ggaccacacg gcgtcccttc gatgctgaac tccaatcttt ctgcaaagtt taccagaaaa 2161 ttattttggt gcccagacca gactatgctt ccagatacgt tctgtggaag caaatCATTG 2221 AACGCAATGG AGGAGTCCTC ACCAGTGCCT TGAATGTCAG CTGCCTGGCG AAGGTCACTG 2281 ACGGCTTCAC CCAAGGACAT ATAGTCGAAG TGGTTAAAGG CGTGCTCACA GATCAGAGAA 2341 TCCGGCGGCA AATTCATAAA CCTCTCACTG CAGTTGAGTT TATTACGGCG ATAACCAGCA 2401 TGAATCCAGT GTACAAAGAG GAAGAAGAGA GCTTTAAGAA CTGGTATGCC AAAACTCCTC 2461 TGGGTAAAAA ACGTGCCTTG GCGATAACAG GAGGCAGCAC AGAAAAGGCA AAGGACAAAG 2521 GGAAAAGGAA ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2581 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2641 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAT ATTGTAAGCC ACCTATCACC 2701 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t