Construct: ORF TRCN0000472214

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002237.1_s317c1
Derived from:
ccsbBroadEn_04124
DNA Barcode:
TATATTGTAAGCCACCTATCACCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IQCA1 (79781)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472214
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79781 IQCA1 IQ motif containing with AA... NM_024726.5 100% 100%
2 human 79781 IQCA1 IQ motif containing with AA... XM_011511865.3 99.8% 99.8% 1411_1413delAAG
3 human 79781 IQCA1 IQ motif containing with AA... XM_011511864.2 99% 98.7% 4A>T;7_27del;31_32delAGinsGC
4 human 79781 IQCA1 IQ motif containing with AA... NM_001270585.1 98.9% 98.6% (many diffs)
5 human 79781 IQCA1 IQ motif containing with AA... NM_001270584.1 95% 95% 1009_1010ins123
6 human 79781 IQCA1 IQ motif containing with AA... XM_017004960.1 94% 93.8% (many diffs)
7 human 79781 IQCA1 IQ motif containing with AA... XM_011511866.1 93.9% 93.7% (many diffs)
8 human 79781 IQCA1 IQ motif containing with AA... NR_073043.2 75.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2532
ORF length:
2466
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc gaacgcaatg tataataaga tgtggcatca gacccaagaa gccctcggtg
121ctttactcga taaagagcct cagaagatga ttgaaccaca aagaaatcag gttttcatct
181ttcaaacatt agccaccttc tacgtaaagt atgtgcagat ctttagaaac ctagagaatg
241tctacgacca gttcgtccac ccccagaaac gaatactgat caggaaagtc ctggacgggg
301tgatgggccg catcctggag ctgaagaacg agatggtgga gctggaactc acggagttcc
361attatttcga tgatatcctg caggatctca agctggcccc ccaacaatta gatattccca
421tacccaagta ttttttgaag gagaagttgg aagtaataaa aggaagagag aaaattcttg
481ctcaaatatt agcggacagc ggaatagata catctgacat gaaataccct gtaaagagta
541tccctttcga tgaggctgtt aaattaatcc agattgctga gagggcacgg caaggtcgcc
601taagggcttt attcatgaag caaatctatc tgcaagaata tagagcaaag caatccaaga
661tgcttggcaa gaaagtgaca gatacctggg ctgctgcact ccgcattcag aaggtttggc
721gacgtttcca tcaacgtaag gaaactgaaa aactgagaga agaggagatg atcttcctgg
781gtatgaatcc acctcctctc tttaatgaag tcagtgctac agtaatccag gctgaaaagg
841tggaccgcct gcggaatgag gtgcagataa agcatgaaga ggactacagg gaagccctgg
901ttaccatcaa gaatgaccta aagttgatag aaggcgtgga tatcaaggag aaccttcaag
961accagatccg gcattggttc atcgaatgca gaaatttaac cgggacattt cctgactacc
1021ctgacgttga agaaggaggg tcagctatta ttttttctga caagaccata caacaggtta
1081ttgaggatat catagcaaac caagaggaag aagaaaaaaa caaaaagaag aagaagaaaa
1141aggaaaaaca acccaagaaa gccaaaaaac aaaagaaagg aacaaaggag aaaaataagg
1201aagaagacga aaaatggaaa atgtcaccaa gtctttttct tcctgcaatg aaggaaggat
1261gtaacgcata caaagaaatc tggatgaaaa aagatgagtc ttggaatttc tctcaggact
1321atgatccaga actgatcaaa gaggagaaac gaaaagaatt acagtcagag atcaggatac
1381aggttgatga gttgatgaga caggaactta aaaacttaaa gctagctgtg gacagagaaa
1441gggagcgccc agtgaaagca ggaaagaaaa aggacaagaa aggaaagaaa ggcaaaaaga
1501aagagaagaa ggcaaagaag gataaagatc tgacagctga caggaccatc gagtctctgt
1561ataaggaact ggtggaagaa ggattactga tccaggctct gaaagtcaac ctctctgatt
1621acattggtga gtacagctac ctggggacta ctcttcgcca ggtgtccata gaacccatgc
1681cctccctcct ggatgtcaga cagctcatca cattgtacgg aatctggcca ttaggttctg
1741cagcagtgca tgagaaagct cctttggtga aatcgctact gttagccggg ccgtctgggg
1801tagggaagaa aatgctggtc catgccatct gcaccgaaac gggagccaac ctcttcaact
1861tgtcctcctc taacattgct gggaaatacc ctggcaaaaa tggcctccaa atgatgctgc
1921atgcagtctt caaggtggct cgacagctcc agccctccgt ggtgtggatt gaagacacag
1981aaaaaacctt ctacaaaaaa gttcccaacg cagaaaagat gaatgaacct aaacgcctga
2041aaaaacacct tccccaaatc ctgaaactcc tgaaaccaga tgaccggatt ctgattgtgg
2101ggaccacacg gcgtcccttc gatgctgaac tccaatcttt ctgcaaagtt taccagaaaa
2161ttattttggt gcccagacca gactatgctt ccagatacgt tctgtggaag caaatCATTG
2221AACGCAATGG AGGAGTCCTC ACCAGTGCCT TGAATGTCAG CTGCCTGGCG AAGGTCACTG
2281ACGGCTTCAC CCAAGGACAT ATAGTCGAAG TGGTTAAAGG CGTGCTCACA GATCAGAGAA
2341TCCGGCGGCA AATTCATAAA CCTCTCACTG CAGTTGAGTT TATTACGGCG ATAACCAGCA
2401TGAATCCAGT GTACAAAGAG GAAGAAGAGA GCTTTAAGAA CTGGTATGCC AAAACTCCTC
2461TGGGTAAAAA ACGTGCCTTG GCGATAACAG GAGGCAGCAC AGAAAAGGCA AAGGACAAAG
2521GGAAAAGGAA ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
2581ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
2641CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAT ATTGTAAGCC ACCTATCACC
2701TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)