Construct: ORF TRCN0000472215
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014787.1_s317c1
- Derived from:
- ccsbBroadEn_02268
- DNA Barcode:
- AGCCGACTAAACAGTACAAATATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- G3BP2 (9908)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472215
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9908 | G3BP2 | G3BP stress granule assembl... | NM_203504.2 | 100% | 100% | |
| 2 | human | 9908 | G3BP2 | G3BP stress granule assembl... | XM_017008878.1 | 100% | 100% | |
| 3 | human | 9908 | G3BP2 | G3BP stress granule assembl... | XM_017008879.1 | 100% | 100% | |
| 4 | human | 9908 | G3BP2 | G3BP stress granule assembl... | NM_012297.4 | 93.1% | 93.1% | 725_823del |
| 5 | human | 9908 | G3BP2 | G3BP stress granule assembl... | NM_203505.2 | 93.1% | 93.1% | 725_823del |
| 6 | human | 9908 | G3BP2 | G3BP stress granule assembl... | XM_005263382.3 | 93.1% | 93.1% | 725_823del |
| 7 | human | 9908 | G3BP2 | G3BP stress granule assembl... | XM_005263383.3 | 93.1% | 93.1% | 725_823del |
| 8 | human | 9908 | G3BP2 | G3BP stress granule assembl... | XM_011532441.2 | 93.1% | 93.1% | 725_823del |
| 9 | human | 9908 | G3BP2 | G3BP stress granule assembl... | XM_017008876.1 | 93.1% | 93.1% | 725_823del |
| 10 | human | 9908 | G3BP2 | G3BP stress granule assembl... | XM_024454294.1 | 93.1% | 93.1% | 725_823del |
| 11 | mouse | 23881 | G3bp2 | GTPase activating protein (... | NM_001080795.2 | 92.7% | 98.2% | (many diffs) |
| 12 | mouse | 23881 | G3bp2 | GTPase activating protein (... | NM_001080796.2 | 92.7% | 98.2% | (many diffs) |
| 13 | mouse | 23881 | G3bp2 | GTPase activating protein (... | NM_001080794.2 | 86.3% | 91.4% | (many diffs) |
| 14 | mouse | 23881 | G3bp2 | GTPase activating protein (... | NM_001080797.2 | 86.3% | 91.4% | (many diffs) |
| 15 | mouse | 23881 | G3bp2 | GTPase activating protein (... | NM_011816.4 | 86.3% | 91.4% | (many diffs) |
| 16 | mouse | 23881 | G3bp2 | GTPase activating protein (... | XM_006534927.3 | 86.3% | 91.4% | (many diffs) |
| 17 | mouse | 23881 | G3bp2 | GTPase activating protein (... | XM_006534928.3 | 86.3% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1413
- ORF length:
- 1347
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt tatggagaag cccagtccgc tgcttgtagg gcgggagttt gtgaggcaat 121 attatacttt gctgaataaa gctccggaat atttacacag gttttatggc aggaattctt 181 cctatgttca tggtggagta gatgctagtg gaaagcccca ggaagctgtt tatggccaaa 241 atgatataca ccacaaagta ttatctctga acttcagtga atgtcatact aaaattcgtc 301 atgtggatgc tcatgcaacc ttgagtgatg gagtagttgt ccaggtcatg ggtttgctgt 361 ctaacagtgg acaaccagaa agaaagttta tgcaaacctt tgttctggct cctgaaggat 421 ctgttccaaa taaattttat gttcacaatg atatgtttcg ttatgaagat gaagtgtttg 481 gtgattctga gcctgaactt gatgaagaat cagaagatga agtagaagag gaacaagaag 541 aaagacaacc atctcctgaa cctgtgcaag aaaatgctaa cagtggttac tatgaagctc 601 accctgtgac taatggcata gaggagcctt tggaagaatc ctctcatgaa cctgaacctg 661 agccagaatc tgaaacaaag actgaagagc tgaaaccaca agtggaggag aagaacttag 721 aagaactaga ggagaaatct actactcctc ctccggcaga acctgtttct ctgccacaag 781 aaccaccaaa gccaagagtc gaagctaaac cagaagttca atctcagcca cctcgtgtgc 841 gtgaacaacg acctagagaa cgacctggtt ttcctcctag aggaccaaga ccaggcagag 901 gagatatgga acagaatgac tctgacaacc gtagaataat tcgctatcca gatagtcatc 961 aactttttgt tggtaacttg ccacatgata ttgatgaaaa tgagctaaag gaattcttca 1021 tgagttttgg aaacgttgtg gaacttcgca tcaataccaa gggtgttggg ggaaagcttc 1081 caaattttgg ttttgtggtt tttgatgact ctgaaccagt tcagagaatc ttaattgcaa 1141 aaccgattat gtttcgaggg gaagtacgtt taaatgtgga agagaaaaaa acaagagcTG 1201 CAAGAGAGCG AGAAACCAGA GGTGGTGGTG ATGATCGCAG GGATATTAGG CGCAATGATC 1261 GAGGTCCCGG TGGTCCACGT GGAATTGTGG GTGGTGGAAT GATGCGTGAT CGTGATGGAA 1321 GAGGACCTCC TCCAAGGGGT GGCATGGCAC AGAAACTTGG CTCTGGAAGA GGAACCGGGC 1381 AAATGGAGGG CCGCTTCACA GGACAGCGTC GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1441 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1501 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 1561 CCGACTAAAC AGTACAAATA TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1621 ttgtgaaaga tt