Construct: ORF TRCN0000472225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016354.1_s317c1
- Derived from:
- ccsbBroadEn_12188
- DNA Barcode:
- CACTTCCGCTATCCATCCCTAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBA6 (55236)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472225
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55236 | UBA6 | ubiquitin like modifier act... | XM_017008359.2 | 37.9% | 36.4% | (many diffs) |
| 2 | human | 55236 | UBA6 | ubiquitin like modifier act... | NM_018227.6 | 36.3% | 35% | (many diffs) |
| 3 | human | 55236 | UBA6 | ubiquitin like modifier act... | XR_001741275.2 | 17.9% | (many diffs) | |
| 4 | mouse | 231380 | Uba6 | ubiquitin-like modifier act... | NM_172712.2 | 31.7% | 29.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1233
- ORF length:
- 1167
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggtatgga aggatctgag cctgtggccg cccatcaggg ggaagaggcg tcctgttctt 121 cctgggggac tggcagcaca aataaaaatt tgcccattat gtcaacagca tctgtggaaa 181 tcgatgatgc attgtatagt cgacagaggt acgttcttgg agacacagca atgcagaaga 241 tggccaagtc ccatgttttc ttaagtggga tgggtggtct tggtttggaa attgcaaaga 301 atcttgttct tgcagggatt aaggcagtta caattcatga tacagaaaaa tgccaagcat 361 gggatctagg aaccaacttc tttctcagtg aagatgatgt tgttaataag agaaacaggg 421 ctgaagctgt acttaaacat attgcagaac taaatccata cgttcatgtc acatcatctt 481 ctgttccttt caatgagacc acagatctct cctttttaga taaataccag tgtgtagtat 541 tgactgagat gaaacttcca ttgcagaaga agatcaatga cttttgccgt tctcagtgcc 601 ctccaattaa gtttatcagt gcagatgtac atggaatttg gtcaaggtta ttttgtgatt 661 tcggtgatga atttgaagtt ttagatacaa caggagaaga accaaaagaa attttcattt 721 caaacataac gcaagcaaat cctGGCATTG TTACTTGCCT TGAAAATCAT CCTCACAAAC 781 TGGAGACAGG ACAATTCCTA ACATTTCGAG AAATTAATGG AATGACAGGT TTAAATGGAT 841 CTATACAACA AATAACGGTG ATATCGCCAT TTTCTTTTAG TATTGGTGAC ACCACAGAAC 901 TGGAACCATA TTTACATGGA GGCATAGCTG TCCAAGTTAA GACTCCTAAA ACAGTTTTTT 961 TTGAATCACT GGAGAGGCAG TTAAAACATC CAAAGTGCCT TATTGTGGAT TTTAGCAACC 1021 CTGAGGCACC TTTAGAGATT CACACAGCTA TGCTTGCCTT GGACCAGTTT CAGGAGAAAT 1081 ACAGTCGCAA GCCAAATGTT GGATGCCAAC AAGATTCAGA AGAACTGTTG AAACTAGCAA 1141 CATCTATAAG TGAAACCTTG GAAGAGAAGG TGACTATTGA AATTTATGGC TGTCCGAATA 1201 TTTGTTTGTT AATACATAAG TGTTCTGTAT ATTACCCAAC TTTCTTGTAC AAAGTGGTTG 1261 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1321 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 1381 CTTCCGCTAT CCATCCCTAA AGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1441 ttgtgaaaga tt