Construct: ORF TRCN0000472249
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007536.1_s317c1
- Derived from:
- ccsbBroadEn_02029
- DNA Barcode:
- GACTCGTCGCGGCACAGCATGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GGH (8836)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472249
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8836 | GGH | gamma-glutamyl hydrolase | NM_003878.3 | 100% | 100% | |
| 2 | human | 8836 | GGH | gamma-glutamyl hydrolase | XM_011517623.3 | 81.3% | 72.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagtccgggc tgcctgctgt gcgtgctggg cctgctactc tgcggggcgg 121 cgagcctcga gctgtctaga ccccacggcg acaccgccaa gaagcccatc atcggaatat 181 taatgcaaaa atgccgtaat aaagtcatga aaaactatgg aagatactat attgctgcgt 241 cctatgtaaa gtacttggag tctgcaggtg cgagagttgt accagtaagg ctggatctta 301 cagagaaaga ctatgaaata cttttcaaat ctattaatgg aatccttttc cctggaggaa 361 gtgttgacct cagacgctca gattatgcta aagtggccaa aatattttat aacttgtcca 421 tacagagttt tgatgatgga gactattttc ctgtgtgggg cacatgcctt ggatttgaag 481 agctttcact gctgattagt ggagagtgct tattaactgc cacagatact gttgacgtgg 541 caatgccgct gaacttcact ggaggtcaat tgcacagcag aatgttccag aattttccta 601 ctgagttgtt gctgtcatta gcagtagaac ctctgactgc caatttccat aagtggagcc 661 tctccgtgaa GAATTTTACA ATGAATGAAA AGTTAAAGAA GTTTTTCAAT GTCTTAACTA 721 CAAATACAGA TGGCAAGATT GAGTTTATTT CAACAATGGA AGGATATAAG TATCCAGTAT 781 ATGGTGTCCA GTGGCATCCA GAGAAAGCAC CTTATGAGTG GAAGAATTTG GATGGCATTT 841 CCCATGCACC TAATGCTGTG AAAACCGCAT TTTATTTAGC AGAGTTTTTT GTTAATGAAG 901 CTCGGAAAAA CAACCATCAT TTTAAATCTG AATCTGAAGA GGAGAAAGCA TTGATTTATC 961 AGTTCAGTCC AATTTATACT GGAAATATTT CTTCATTTCA GCAATGTTAC ATATTTGATT 1021 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGAGACTC GTCGCGGCAC AGCATGTTCA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt