Construct: ORF TRCN0000472285
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000388.1_s317c1
- Derived from:
- ccsbBroadEn_13625
- DNA Barcode:
- ACACGACGCCCATAGGCCCGACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NHLRC2 (374354)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472285
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 374354 | NHLRC2 | NHL repeat containing 2 | NM_198514.4 | 50.5% | 50.5% | 1_1077del |
| 2 | human | 374354 | NHLRC2 | NHL repeat containing 2 | XM_011539769.3 | 38.9% | 38.9% | 1_1077del;1925_1926delTAinsGC;1929_1929delCins250 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1167
- ORF length:
- 1101
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agggactcat cagatatggg cactcctgct ggactctggc aaactgccaa 121 agaaaaatga gttaacaaaa ggaacctgcc ttaggtttgc tggaagtgga aatgaagaga 181 atcgaaacaa tgcctatcct cacaaggcag gttttgccca accttcaggc ctttccttgg 241 cctctgaaga tccctggagc tgcttgtttg tagcagatag tgagagcagt acagtgagaa 301 ccgtttcact gaaagatgga gcagtgaagc acctcgtagg aggagaaaga gaccccatga 361 atttatttgc ttttggtgat gttgatggag taggaatcaa tgcaaagctt caacaccccc 421 ttggagtaac atgggacaaa aaaaggaatt tactttatgt tgcagactcc tacaatcaca 481 agattaaagt tgtggatcca aaaacaaaaa actgtacaac attagcagga actggagaca 541 caaataatgt taccagttcc agttttacag agtcaacttt taatgaaCCA GGAGGCTTGT 601 GTATTGGAGA GAATGGAGAA TTATTATATG TAGCAGACAC CAATAATCAT CAAATTAAAG 661 TGATGGATTT AGAAACTAAA ATGGTATCTG TGCTCCCCAT CTTCAGATCT GAAAATGCTG 721 TGGTAGATGG CCCGTTCCTA GTAGAAAAAC AGAAGACATT ACCCAAACTA CCTAAATCTG 781 CTCCAAGCAT TAGGCTTTCC CCCGTGACTG CGTGTGCTGG CCAGACTCTT CAGTTCAAAC 841 TCAGATTAGA CCTCCCATCA GGATCAAAGC TAACTGAAGG AGTATCCAGT TGCTGGTTTC 901 TAACAGCTGA AGGCAATGAA TGGCTACTTC AAGGACAGAT AGCAGCTGGA GATATAGAGA 961 ACATTTCCAG TCAACCAACA ATTTCACTAC AAATTCCTGA TGATTGCTTA TCACTTGAAG 1021 CCATTGTATC TGTCAGTGTG TTTCTTTATT ACTGTAGTGC AGACAGCAGT GCTTGTATGA 1081 TGAAGGCAAT TTTGTTCAGT CAGCCTTTAC AAATAACGGA TACACAGCAA GGTTGCATAG 1141 CTCCAGTAGA GCTCAGGTAT GTATTTTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1201 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1261 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACACGACG 1321 CCCATAGGCC CGACGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1381 aagatt