Construct: ORF TRCN0000472303
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012969.1_s317c1
- Derived from:
- ccsbBroadEn_09681
- DNA Barcode:
- CAGCCGAGGTCGAGTGATCTGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BTLA (151888)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472303
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151888 | BTLA | B and T lymphocyte associated | NM_001085357.2 | 99.8% | 99.5% | 656C>T |
| 2 | human | 151888 | BTLA | B and T lymphocyte associated | NM_181780.4 | 83.2% | 83% | 402_545del;800C>T |
| 3 | human | 151888 | BTLA | B and T lymphocyte associated | XM_017005748.2 | 83.2% | 83% | 402_545del;800C>T |
| 4 | human | 151888 | BTLA | B and T lymphocyte associated | XM_011512447.3 | 81.5% | 81.3% | 402_563del;818C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 792
- ORF length:
- 723
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagacattg cctgccatgc ttggaactgg gaaattattt tgggtcttct 121 tcttaatccc atatctggac atctggaaca tccatgggaa agaatcatgt gatgtacagc 181 tttatataaa gagacaatct gaacactcca tcttagcagg agatcccttt gaactagaat 241 gccctgtgaa atactgtgct aacaggcctc atgtgacttg gtgcaagctc aatggaacaa 301 catgtgtaaa acttgaagat agacaaacaa gttGGAAGGA AGAGAAGAAC ATTTCATTTT 361 TCATTCTACA TTTTGAACCA GTGCTTCCTA ATGACAATGG GTCATACCGC TGTTCTGCAA 421 ATTTTCAGTC TAATCTCATT GAAAGCCACT CAACAACTCT TTATGTGACA GGAAAGCAAA 481 ATGAACTCTC TGACACAGCA GGAAGGGAAA TTAACCTGGT TGATGCTCAC CTTAAGAGTG 541 AGCAAACAGA AGCAAGCACC AGGCAAAATT CCCAAGTACT GCTATCAGAA ACTGGAATTT 601 ATGATAATGA CCCTGACCTT TGTTTCAGGA TGCAGGAAGG GTCTGAAGTT TATTCTAATC 661 CATGCCTGGA AGAAAACAAA CCAGGCATTG TTTATGCTTC CCTGAACCAT TCTGTCATTG 721 GACTGAACTC AAGACTGGCA AGAAATGTAA AAGAAGCACC AACAGAATAT GCATCCATAT 781 GTGTGAGGAG TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAG CCGAGGTCGA GTGATCTGGT 961 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t