Transcript: Human NM_001085357.2

Homo sapiens B and T lymphocyte associated (BTLA), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
BTLA (151888)
Length:
2982
CDS:
115..840

Additional Resources:

NCBI RefSeq record:
NM_001085357.2
NBCI Gene record:
BTLA (151888)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001085357.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000435139 CAGAATAGTAGAACGTTTAAA pLKO_005 1092 3UTR 100% 15.000 21.000 N BTLA n/a
2 TRCN0000435951 TCATACCGCTGTTCTGCAAAT pLKO_005 448 CDS 100% 10.800 15.120 N BTLA n/a
3 TRCN0000149495 GAACTAGAATGCCCTGTGAAA pLKO.1 277 CDS 100% 4.950 6.930 N BTLA n/a
4 TRCN0000148370 CACAGCAGGAAGGGAAATTAA pLKO.1 540 CDS 100% 15.000 12.000 N BTLA n/a
5 TRCN0000413967 TTTGACTAACCAACCATAAAT pLKO_005 1242 3UTR 100% 15.000 10.500 N BTLA n/a
6 TRCN0000422741 TGTTAGTGCTTGGGTCTTAAG pLKO_005 961 3UTR 100% 10.800 7.560 N BTLA n/a
7 TRCN0000183326 GAGATCCCTTTGAACTAGAAT pLKO.1 266 CDS 100% 5.625 3.938 N BTLA n/a
8 TRCN0000147179 CATGGGAAAGAATCATGTGAT pLKO.1 199 CDS 100% 4.950 3.465 N BTLA n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001085357.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09681 pDONR223 100% 99.8% 99.5% None 656C>T n/a
2 ccsbBroad304_09681 pLX_304 0% 99.8% 99.5% V5 656C>T n/a
3 TRCN0000472303 CAGCCGAGGTCGAGTGATCTGGTA pLX_317 62.8% 99.8% 99.5% V5 656C>T n/a
Download CSV