Construct: ORF TRCN0000472309
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007656.1_s317c1
- Derived from:
- ccsbBroadEn_01619
- DNA Barcode:
- GTATATTTGAAATTTCTATCAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STXBP3 (6814)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472309
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6814 | STXBP3 | syntaxin binding protein 3 | NM_007269.4 | 100% | 100% | |
| 2 | mouse | 20912 | Stxbp3 | syntaxin binding protein 3 | NM_011504.1 | 87.7% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1842
- ORF length:
- 1776
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gccgccggtg gcagagaggg ggctaaagag cgtcgtgtgg cagaagataa 121 aagcaacagt gtttgatgac tgcaagaaag aaggcgaatg gaagataatg cttttagatg 181 aatttaccac taagcttttg gcatcgtgtt gcaaaatgac agatcttcta gaagaaggta 241 ttactgttgt agagaatatt tataagaacc gtgaacctgt cagacaaatg aaagctcttt 301 atttcatcac tccgacatca aagtctgtag attgtttctt acatgatttt gcaagtaaat 361 cggagaacaa gtataaagca gcatatattt acttcactga cttttgccct gataatctct 421 ttaacaaaat taaggcttct tgctccaagt caataagaag atgtaaagaa ataaatattt 481 ccttcattcc acatgaatct caggtgtata ctcttgatgt accagatgca ttctattact 541 gttatagtcc agaccctggt aatgcaaagg gaaaagatgc cattatggaa acaatggctg 601 accagatagt tacagtgtgt gccaccttgg atgaaaatcc cggagtaaga tataaaagta 661 aacctctaga taatgccagt aagcttgcac agcttgttga aaaaaagctt gaagactact 721 acaagattga tgaaaagagc ctaataaagg gtaaaactca ttcacagctc ttaataattg 781 atcgtggctt tgatcctgtg tccactgtcc tgcatgaact gacctttcag gcaatggcat 841 atgatctact accaattgag aatgatacat acaaatataa aacagatgga aaagaaaagg 901 aggccatcct tgaagaagaa gatgacctct gggttagaat tcgacatcga catattgcgg 961 ttgtgttaga ggaaattccc aagcttatga aagaaatttc atcaacaaag aaagcaacag 1021 aaggaaagac atcacttagt gctcttaccc agctgatgaa aaagatgccc catttccgaa 1081 aacagattac taagcaagtt gtccatctta acttagcaga agattgcatg aataagttca 1141 agcttaatat agaaaagctc tgcaaaactg aacaggacct ggcacttgga actgatgcag 1201 aaggacagaa ggtgaaagat tccatgcgag tactccttcc agttctactc aacaaaaatc 1261 atgataattg tgataaaata agagcaattc tactttatat cttcagtatt aatggaacta 1321 cggaagaaaa tttggacagg ttgatccaga atgtaaagat agaaaatgag agtgacatga 1381 ttcgtaactG GAGTTACCTT GGTGTTCCCA TTGTTCCCCA ATCTCAACAA GGCAAACCGT 1441 TAAGAAAGGA TCGGTCTGCA GAAGAAACTT TTCAGCTCTC TCGGTGGACA CCTTTTATCA 1501 AAGATATTAT GGAGGATGCT ATTGATAATA GATTAGATTC AAAAGAATGG CCATATTGTT 1561 CCCAGTGTCC AGCAGTATGG AATGGTTCAG GAGCTGTAAG TGCTCGCCAG AAACCCAGAG 1621 CTAATTATTT AGAAGACCGA AAAAATGGGT CAAAGCTGAT TGTTTTTGTA ATTGGAGGGA 1681 TCACATACTC TGAAGTGCGT TGTGCTTATG AAGTTTCTCA GGCACATAAA TCCTGTGAAG 1741 TTATTATTGG TTCTACACAT GTTTTAACAC CCAAAAAGCT GTTGGATGAT ATAAAGATGC 1801 TGAATAAACC CAAGGATAAA GTCTCCTTAA TTAAAGATGA ATACCCAACT TTCTTGTACA 1861 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1921 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1981 AGGACGAGTA TATTTGAAAT TTCTATCAAC TACGCGTTAA GTCgacaatc aacctctgga 2041 ttacaaaatt tgtgaaagat t