Construct: ORF TRCN0000472315
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017929.1_s317c1
- Derived from:
- ccsbBroadEn_03126
- DNA Barcode:
- CACCAAGAACGTCGCTTATTACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PACSIN1 (29993)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472315
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29993 | PACSIN1 | protein kinase C and casein... | NM_001199583.2 | 100% | 100% | |
| 2 | human | 29993 | PACSIN1 | protein kinase C and casein... | NM_020804.5 | 100% | 100% | |
| 3 | human | 29993 | PACSIN1 | protein kinase C and casein... | XM_011514541.1 | 100% | 100% | |
| 4 | mouse | 23969 | Pacsin1 | protein kinase C and casein... | NM_001286743.1 | 89.1% | 95% | (many diffs) |
| 5 | mouse | 23969 | Pacsin1 | protein kinase C and casein... | NM_001286744.1 | 89.1% | 95% | (many diffs) |
| 6 | mouse | 23969 | Pacsin1 | protein kinase C and casein... | NM_011861.3 | 89.1% | 95% | (many diffs) |
| 7 | mouse | 23969 | Pacsin1 | protein kinase C and casein... | NM_178365.4 | 89.1% | 95% | (many diffs) |
| 8 | mouse | 23969 | Pacsin1 | protein kinase C and casein... | XM_006524226.3 | 89.1% | 95% | (many diffs) |
| 9 | mouse | 23969 | Pacsin1 | protein kinase C and casein... | XM_011246431.2 | 89.1% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1398
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cagctcctac gatgaggcct cactggcgcc agaggagacc accgacagct 121 tctgggaggt ggggaactac aagcggaccg tgaagcgcat cgatgacggc caccgtctat 181 gcaacgacct gatgaactgc gtgcaggagc gcgccaagat cgagaaggcg tacgggcagc 241 agctcaccga ctgggccaag cgttggcgcc agctcatcga gaaaggccca cagtatggca 301 gcctggagcg ggcctggggt gccataatga cagaggcaga caaggtgagc gagctgcacc 361 aggaggtgaa gaacaatctg ctgaatgagg acctggagaa ggtgaagaac tggcagaagg 421 acgcctatca caagcagatc atgggtggct tcaaggagac gaaggaggct gaagatggct 481 tccgcaaggc ccagaagcct tgggccaaga agatgaagga gctggaggca gccaagaagg 541 cctaccattt ggcttgcaaa gaggaaaagc tggccatgac acgggagatg aacagcaaga 601 cggagcaatc ggtcacacct gagcagcaaa agaagctgca ggacaaagtg gacaagtgca 661 agcaggatgt gcagaagaca caggagaagt atgagaaagt gctggaagat gtgggcaaga 721 ccacacccca gtacatggag aacatggagc aggtgtttga gcaatgccag caatttgagg 781 aaaagcggct ggtcttcctc aaggaggtgc tgctggacat caaacggcac ctcaacctgg 841 ctgagaacag cagctacatc catgtgtacc gtgagctgga gcaggccatc cggggggctg 901 atgcccagga agaccTCAGA TGGTTCCGCA GCACCAGTGG CCCCGGCATG CCCATGAACT 961 GGCCCCAGTT TGAGGAGTGG AACCCAGACC TTCCTCACAC CACCACCAAG AAGGAGAAAC 1021 AGCCTAAGAA GGCAGAGGGA GTGGCGCTGA CCAATGCCAC TGGGGCGGTA GAGTCCACAT 1081 CCCAGGCTGG GGACCGCGGC AGTGTTAGCA GCTACGACAG AGGCCAGCCC TACGCCACCG 1141 AGTGGTCAGA CGACGAGAGT GGGAACCCCT TTGGGGGCAG TGAGACCAAC GGGGGCGCCA 1201 ACCCCTTTGA GGACGACTCC AAGGGAGTGC GCGTGCGGGC ACTCTACGAC TATGACGGCC 1261 AGGAGCAGGA CGAGCTCAGC TTTAAGGCCG GAGACGAACT CACCAAGCTG GGCGAGGAGG 1321 ATGAGCAGGG CTGGTGCCGT GGGCGGCTGG ACAGCGGGCA GCTGGGCCTC TACCCTGCCA 1381 ACTACGTGGA GGCTATCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1441 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1501 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACACCAAG AACGTCGCTT 1561 ATTACGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt