Construct: ORF TRCN0000472317
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013010.1_s317c1
- Derived from:
- ccsbBroadEn_08193
- DNA Barcode:
- GCTCAGACAAGGTAGCATAACGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC35B3 (51000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472317
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51000 | SLC35B3 | solute carrier family 35 me... | NM_001142540.1 | 99.9% | 100% | 1098T>C |
| 2 | human | 51000 | SLC35B3 | solute carrier family 35 me... | NM_001142541.2 | 99.9% | 100% | 1098T>C |
| 3 | human | 51000 | SLC35B3 | solute carrier family 35 me... | NM_001370476.2 | 99.9% | 100% | 1098T>C |
| 4 | human | 51000 | SLC35B3 | solute carrier family 35 me... | NM_015948.4 | 99.9% | 100% | 1098T>C |
| 5 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XM_017010910.1 | 99.9% | 100% | 1098T>C |
| 6 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XM_017010911.1 | 99.9% | 100% | 1098T>C |
| 7 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XM_017010912.2 | 92.1% | 92.2% | 779_780ins93;1005T>C |
| 8 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XM_017010913.1 | 92.1% | 92.2% | 779_780ins93;1005T>C |
| 9 | human | 51000 | SLC35B3 | solute carrier family 35 me... | NM_001370478.2 | 91.9% | 92% | 0_1ins96;1002T>C |
| 10 | human | 51000 | SLC35B3 | solute carrier family 35 me... | NM_001370479.1 | 91.9% | 92% | 0_1ins96;1002T>C |
| 11 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XM_011514648.1 | 91.9% | 92% | 0_1ins96;1002T>C |
| 12 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XM_017010914.1 | 91.9% | 92% | 0_1ins96;1002T>C |
| 13 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XM_006715103.3 | 91.7% | 91.5% | (many diffs) |
| 14 | human | 51000 | SLC35B3 | solute carrier family 35 me... | NM_001370477.1 | 84.2% | 84.2% | 0_1ins96;683_684ins93;909T>C |
| 15 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XM_011514650.2 | 61% | 57% | (many diffs) |
| 16 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XR_001743449.1 | 59.1% | (many diffs) | |
| 17 | human | 51000 | SLC35B3 | solute carrier family 35 me... | NR_163441.1 | 57.2% | (many diffs) | |
| 18 | human | 51000 | SLC35B3 | solute carrier family 35 me... | NR_163442.1 | 56.4% | (many diffs) | |
| 19 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XR_001743446.1 | 56% | (many diffs) | |
| 20 | human | 51000 | SLC35B3 | solute carrier family 35 me... | NR_109913.1 | 54.3% | (many diffs) | |
| 21 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XR_001743451.1 | 53.4% | (many diffs) | |
| 22 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XR_001743448.1 | 50.4% | (many diffs) | |
| 23 | human | 51000 | SLC35B3 | solute carrier family 35 me... | NR_109914.1 | 45.2% | (many diffs) | |
| 24 | human | 51000 | SLC35B3 | solute carrier family 35 me... | XR_002956285.1 | 43.5% | (many diffs) | |
| 25 | mouse | 108652 | Slc35b3 | solute carrier family 35, m... | NM_001170431.1 | 82.1% | 83% | (many diffs) |
| 26 | mouse | 108652 | Slc35b3 | solute carrier family 35, m... | NM_001170430.1 | 79.5% | 83.5% | (many diffs) |
| 27 | mouse | 108652 | Slc35b3 | solute carrier family 35, m... | NM_134060.4 | 79.5% | 83.5% | (many diffs) |
| 28 | mouse | 108652 | Slc35b3 | solute carrier family 35, m... | XM_017315356.1 | 79.5% | 83.5% | (many diffs) |
| 29 | mouse | 108652 | Slc35b3 | solute carrier family 35, m... | XM_006516527.2 | 76.4% | 77.3% | (many diffs) |
| 30 | mouse | 108652 | Slc35b3 | solute carrier family 35, m... | XM_006516528.2 | 73.8% | 77.5% | (many diffs) |
| 31 | mouse | 108652 | Slc35b3 | solute carrier family 35, m... | XM_006516529.2 | 73.8% | 77.5% | (many diffs) |
| 32 | mouse | 108652 | Slc35b3 | solute carrier family 35, m... | XM_006516530.2 | 73.8% | 77.5% | (many diffs) |
| 33 | mouse | 108652 | Slc35b3 | solute carrier family 35, m... | XM_006516531.2 | 55.1% | 58.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1269
- ORF length:
- 1203
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cttgacacag caagcaaaag acatacagaa cataacagtc caggaaacca 121 acaaaaataa ctctgaaagc attgaatgca gcaaaataac aatggatctc aagttcaaca 181 attccaggaa atatatttct atcactgtgc catccaaaac ccaaacaatg tcaccacaca 241 tcaagtcagt tgacgacgtt gtggtacttg gcatgaatct cagcaagttt aacaaactta 301 ctcagttttt catatgtgtt gctggagttt ttgtatttta cctaatttat gggtatttac 361 aggaattaat attttcagtg gagggtttta agtcctgtgg ctggtacctt accttagtgc 421 agtttgcctt ttactccata tttggcctaa tagaacttca gcttattcag gacaaaagga 481 ggagaatacc aggaaaaacc tacatgataa tagcttttct aactgtgggt actatggggt 541 tatcaaacac ttccttgggc tacctgaatt accctaccca agtcatcttc aagtgctgca 601 aattgattcc tgttatgcta ggaggagttt ttattcaagg aaagcgttat aatgttgcag 661 atgtgtctgc tgccatatgt atgagccttg gcctgatatg gtttaccctc gctgacagca 721 caactgcacc aaatttcaac ctgacgggtg tggtgcttat ttccctggca ctatgtgcag 781 atgccgtcat tGGAAATGTT CAAGAGAAAG CTATGAAACT TCATAATGCT TCTAATTCTG 841 AAATGGTATT GTATTCGTAT TCAATTGGTT TTGTATACAT TTTACTGGGA TTGACATGCA 901 CTAGTGGATT AGGCCCTGCA GTAACATTTT GTGCAAAGAA TCCAGTTCGG ACCTATGGTT 961 ATGCGTTCCT TTTTTCCCTC ACTGGATATT TTGGAATCTC CTTTGTTCTG GCTTTGATTA 1021 AAATTTTTGG TGCACTTATT GCTGTAACAG TGACAACAGG AAGAAAAGCA ATGACCATTG 1081 TACTTTCGTT TATATTCTTT GCTAAACCAT TCACGTTTCA GTATGTATGG TCTGGTTTGT 1141 TAGTTGTCCT TGGTATATTT CTCAATGTTT ACAGCAAAAA TATGGATAAA ATAAGACTAC 1201 CATCACTGTA TGATTTGATA AACAAATCAG TGGAAGCAAG AAAGTCAAGG ACGCTGGCAC 1261 AGACTGTATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1321 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1381 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCTCAG ACAAGGTAGC ATAACGTTAC 1441 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt