Construct: ORF TRCN0000472318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010172.1_s317c1
- Derived from:
- ccsbBroadEn_02889
- DNA Barcode:
- CATTTTATCCGAAACCGGTTGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WWTR1 (25937)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472318
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25937 | WWTR1 | WW domain containing transc... | NM_001168278.2 | 100% | 100% | |
| 2 | human | 25937 | WWTR1 | WW domain containing transc... | NM_001168280.3 | 100% | 100% | |
| 3 | human | 25937 | WWTR1 | WW domain containing transc... | NM_001348362.1 | 100% | 100% | |
| 4 | human | 25937 | WWTR1 | WW domain containing transc... | NM_015472.6 | 100% | 100% | |
| 5 | human | 25937 | WWTR1 | WW domain containing transc... | XM_017006122.1 | 100% | 100% | |
| 6 | mouse | 97064 | Wwtr1 | WW domain containing transc... | NM_133784.3 | 88.1% | 91.2% | (many diffs) |
| 7 | mouse | 97064 | Wwtr1 | WW domain containing transc... | NM_001168281.1 | 77.2% | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1266
- ORF length:
- 1200
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tccggcctcg gcgccccctc cgctcccgcc gcctgggcag caagtgatcc 121 acgtcacgca ggacctagac acagacctcg aagccctctt caactctgtc atgaatccga 181 agcctagctc gtggcggaag aagatcctgc cggagtcttt ctttaaggag cctgattcgg 241 gctcgcactc gcgccagtcc agcaccgact cgtcgggcgg ccacccgggg cctcgactgg 301 ctgggggtgc ccagcatgtc cgctcgcact cgtcgcccgc gtccctgcag ctgggcaccg 361 gcgcgggtgc tgcgggtagc cccgcgcagc agcacgcgca cctccgccag cagtcctacg 421 acgtgaccga cgagctgcca ctgcccccgg gctgggagat gaccttcacg gccactggcc 481 agaggtactt cctcaatcac atagaaaaaa tcaccacatg gcaagaccct aggaaggcga 541 tgaatcagcc tctgaatcat atgaacctcc accctgccgt cagttccaca ccagtgcctc 601 agaggtccat ggcagtatcc cagccaaatc tcgtgatgaa tcaccaacac cagcagcaga 661 tggcccccag taccctgagc cagcagaacc accccactca gaacccaccc gcagggctca 721 tgagtatgcc caatgcgctg accactcagc agcagcagca gcagaaactg cggcttcaga 781 gaaTCCAGAT GGAGAGAGAA AGGATTCGAA TGCGCCAAGA GGAGCTCATG AGGCAGGAAG 841 CTGCCCTCTG TCGACAGCTC CCCATGGAAG CTGAGACTCT TGCCCCAGTT CAGGCTGCTG 901 TCAACCCACC CACGATGACC CCAGACATGA GATCCATCAC TAATAATAGC TCAGATCCTT 961 TCCTCAATGG AGGGCCATAT CATTCGAGGG AGCAGAGCAC TGACAGTGGC CTGGGGTTAG 1021 GGTGCTACAG TGTCCCCACA ACTCCGGAGG ACTTCCTCAG CAATGTGGAT GAGATGGATA 1081 CAGGAGAAAA CGCAGGACAA ACACCCATGA ACATCAATCC CCAACAGACC CGTTTCCCTG 1141 ATTTCCTTGA CTGTCTTCCA GGAACAAACG TTGACTTAGG AACTTTGGAA TCTGAAGACC 1201 TGATCCCCCT CTTCAATGAT GTAGAGTCTG CTCTGAACAA AAGTGAGCCC TTTCTAACCT 1261 GGCTGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACATTTTATC CGAAACCGGT TGTGAACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt