Construct: ORF TRCN0000472321
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006554.1_s317c1
- Derived from:
- ccsbBroadEn_16124
- DNA Barcode:
- CGTAAACGGGGCGCCCAGACCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CERS3 (204219)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472321
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 204219 | CERS3 | ceramide synthase 3 | NM_001290342.2 | 99.5% | 99.4% | 159A>G;428_430delTAA;1108A>G |
| 2 | human | 204219 | CERS3 | ceramide synthase 3 | NM_001290343.2 | 99.5% | 99.4% | 159A>G;428_430delTAA;1108A>G |
| 3 | human | 204219 | CERS3 | ceramide synthase 3 | NM_178842.5 | 99.5% | 99.4% | 159A>G;428_430delTAA;1108A>G |
| 4 | human | 204219 | CERS3 | ceramide synthase 3 | XM_017022003.1 | 99.5% | 99.4% | 159A>G;428_430delTAA;1108A>G |
| 5 | human | 204219 | CERS3 | ceramide synthase 3 | XM_017022004.1 | 99.5% | 99.4% | 159A>G;428_430delTAA;1108A>G |
| 6 | human | 204219 | CERS3 | ceramide synthase 3 | NM_001290341.2 | 96.7% | 96.7% | (many diffs) |
| 7 | human | 204219 | CERS3 | ceramide synthase 3 | XM_011521355.2 | 96.7% | 96.7% | (many diffs) |
| 8 | human | 204219 | CERS3 | ceramide synthase 3 | XM_011521357.2 | 96.7% | 96.7% | (many diffs) |
| 9 | human | 204219 | CERS3 | ceramide synthase 3 | XM_011521358.1 | 96.7% | 96.7% | (many diffs) |
| 10 | human | 204219 | CERS3 | ceramide synthase 3 | XM_017022002.1 | 96.7% | 96.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1212
- ORF length:
- 1146
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ttggacgttt aaagaatggt tctggttgga aagattctgg cttcctccaa 121 caataaagtg gtcagatctt gaggatcacg atggactcgt ctttgtaaaa ccttctcatt 181 tatacgtgac aattccatat gcttttctct tgctgattat caggcgtgta tttgaaaaat 241 ttgttgcttc acctctagca aaatcatttg gcattaaaga gacagttcga aaggttacac 301 caaatactgt cttagagaat tttttcaaac attccacaag gcaaccattg caaactgata 361 tttatggact ggcaaagaag tgtaacttga cggagcgcca ggtggaaaga tggtttagga 421 gtcggcggaa tcaagagagg ccttccaggc tgaagaaatt ccaggaagct tgctggagat 481 ttgcatttta cttgatcact gttgctggaa ttgcgtttct ttatgataaa ccttggctat 541 atgacttatg ggaggtttgg aatggctatc ccaaacagcc cctgctgcca tcccagtact 601 ggtactacat tttagaaatg agtttttatt ggtctctgtt atttagactt ggctttgatg 661 tcaagagaaa ggattttcta gctcatatca tccaccacct ggctgctatt agtctgatga 721 gcttctcttg gtgtgctaat tatattcgca gtgggaccct cgtgatgatt gtacacgatg 781 tggctgacat ttggctggag tctgctaaga tgttttctta tgctGGATGG ACGCAGACCT 841 GTAACACCCT GTTTTTCATC TTCTCCACCA TATTTTTCAT CAGCCGCCTC ATTGTTTTTC 901 CTTTCTGGAT TTTATATTGC ACGCTGATCT TGCCTATGTA TCACCTCGAG CCTTTCTTTT 961 CATACATCTT CCTCAACCTA CAGCTCATGA TCTTGCAGGT CCTTCACCTT TACTGGGGTT 1021 ATTACATCTT GAAGATGCTC AACAGATGTA TATTCATGAA GAGCATCCAG GATGTGAGGA 1081 GTGATGACGA GGATTATGAA GAGGAAGAGG AAGAGGAAGA AGAAGAGGCT ACCAAAGGCA 1141 AAGAGATGGA TTGTTTAAAG AACGGCCTCG GGGCTGAGAG GCACCTCATT CCCAATGGCC 1201 AGCATGGCCA TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1261 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1321 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGT AAACGGGGCG CCCAGACCGT 1381 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t