Construct: ORF TRCN0000472351
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018918.3_s317c1
- Derived from:
- ccsbBroadEn_15196
- DNA Barcode:
- ACTTGTGCAAACTCCCCACCCAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CARD11 (84433)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472351
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84433 | CARD11 | caspase recruitment domain ... | NM_001324281.2 | 98.6% | 21.7% | (many diffs) |
| 2 | human | 84433 | CARD11 | caspase recruitment domain ... | NM_032415.6 | 98.6% | 21.7% | (many diffs) |
| 3 | mouse | 108723 | Card11 | caspase recruitment domain ... | NM_175362.2 | 85.9% | 21.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 834
- ORF length:
- 765
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgactac atggagacgc tgaaggatga agaggacgcc ttgtgggaga 121 atgtggagtg taaccggcac atgctcagcc gctatatcaa ccctgccaag ctcacgccct 181 acctgcgtca gtgtaaggtc attgatgagc aggatgaaga tgaagtgctt aatgccccta 241 tgctgccatc caagatcaac cgagcaggcc ggctgttgga cattctacat accaaggggc 301 aaaggggcta tgtggtcttc ttggagagcc tagaatttta ttacccagaa ctgtacaact 361 tggtgactgg gaaagagccc actcggagat tctccaccat tgtggtggag gaaggccacg 421 agggcctcac gcacttcctg atgaacgagg tcatcaagct gcagcagcag atgaaggcca 481 aggacctgca acgctgcgag ctgctggcca ggttgcggca gctggaggat gagaagaagc 541 agatgacgct gacgcgcgtg gagctgctaa ccttccagga gcggtactac aagatgaagg 601 aagagcggga cagctacaat gacgagctgg tcaaggtgaa ggacgacaac tacaacttag 661 ccatgcgcta cgcacagctc agtgaggaga agaacatggc ggtcatgagg agccgagacc 721 tccaactcga gatcgatcag ctaaagcacc ggttgaataa gatggaggag gaatgtaagc 781 tggagagaaa tcagtctcta aaactgaaga atgacattga aatcggccca agatgagcag 841 tctggaactg gagcggggag aatgaaatgc tgaagaccaa aaaccaggag ctgcagtcca 901 tcatccaggc cgggaagcgc agcctgccag actcagacaa ggccatcctg gacatcttgg 961 aacacgaccg caaggaggcc ctggaggaca ggcaggagct ggtcaacagg atctacaacc 1021 tgcaggagga ggcccgccag gcagaggagc tgcgagacaa gtacctggag gagaaggagg 1081 acctggagct caagtgctcg accctgggaa aggactgtga aatgtacaag caccgcatga 1141 acacggtcat gctgcagctg gaggaggtgg agcgggagcg ggaccaggcc ttccactccc 1201 gagatgaagc tcagacacag tactcgcagt gcttaatcga aaaggacaag tacaggaagc 1261 agatccgcga gctggaggag aagaacgacg agatgaggat cgagatggtg cggcgggagg 1321 cctgcatcgt caacctggag agcaagctgc ggcgcctctc caaggacagc aacaacctgg 1381 accagagtct gcccaggaac ctgccagtaa ccatcatctc tcaggacttt ggggatgcca 1441 gccccaggac caatggtcaa gaagctgacg attcttccac ctcggaggag tcacctgaag 1501 acagcaagta cttcctgccc taccatccgc cccagcgcag gatgaacctg aagggcatcc 1561 agctgcagag agccaaatcc cccatcagcc tgaagcgaac atcagatttt caagccaagg 1621 ggcacgagga agaaggcacg gacgccagcc ctagctcctg cggatctctg cccatcacca 1681 actccttcac caagatgcag cccccccgga gccgcagcag catcatgtca atcaccgccg 1741 agcccccggg aaacgactcc atcgtcagac gctacaagga ggacgcgccc catcgcagca 1801 cagtcgaaga agacaatgac agcggcgggt ttgacgcctt agatctggat gatgacagtc 1861 acgaacgcta ctccttcgga ccctcctcca tccactcctc ctcctcctcc caccaatccg 1921 agggcctgga tgcctacgac ctggagcagg tcaacctcat gttcaggaag ttctctctgg 1981 aaagaccctt ccggccttcg gtcacctctg tggggcacgt gcggggccca gggccctcgg 2041 tgcagcacac gacgctgaat ggcgacagcc tcacctccca gctcaccctg ctggggggca 2101 acgcgcgagg gagcttcgtg cactcggtca agcctggctc tctggccgag aaagccggcc 2161 tccgtgaggg ccaccagctg ctgctgctag aaggctgcat ccgaggcgag aggcagagtg 2221 tcccgttgga cacatgcacc aaagaggaag cccactggac catccagagg tgcagcggcc 2281 ccgtcacgct gcactacaag gtcaaccacg aagggtaccg gaagctggtg aaggacatgg 2341 aggacggcct gatcacatcg ggggactcgt tctacatccg gctgaacctg aacatctcca 2401 gccagctgga cgcctgcacc atgtccctga agtgtgacga tgttgtgcac gtccgtgaca 2461 ccatgtacca ggacaggcac gagtggctgt gcgcgcgggt cgaccctttc acagaccatg 2521 acctggatat gggcaccata cccagctaca gccgagccca gcagctcctc ctggtgaaac 2581 tgcagcgcct gatgcaccga ggcagccggg aggaggtaga cggcacccac cacaccctgc 2641 gggcactccg gaacaccctg cagccggaag aagcgctttc aacaagcgac ccccgggtca 2701 gccccgtttc tcgggagcaa gctccttttt gcccagctcc ttcagttcgt cagcagtcga 2761 gaaaacaagt ataagcggat gaacagcaac gagcgggtcc gcatcatctc ggggagtcgc 2821 tangggagcc tggcccgggt cctcgctgac gcccaccaag ctctgnactg agaagcagga 2881 agagctggac cctgagagcg agctgggcaa gaacctcagc ctcatcccct acagcctggt 2941 acgcgccttc tactgcgagc gccgccggcc cgtgctcttc acacccaccg tgctggccaa 3001 gacgctggtg cagaggctgc tcaactcggg aggtgccatg gagttcacca tctgcaagtc 3061 agatatcgtc acaagagatg agttcctcag aaggcagaag acggagacca tcatctactc 3121 ccgagagaag aaccccaacg cgttcgaatg catcgccCCT GCCAACATTG AAGCTGTGGC 3181 CGCCAAGAAC AAGCACTGCC TGCTGGAGGC TGGGATCGGC TGCACAAGAG ACTTGATCAA 3241 GTCCAACATC TACCCCATCG TGCTCTTCAT CCGGGTGTGT GAGAAGAACA TCAAGAGGTT 3301 CAGAAAGCTG CTGCCCCGAC CTGAGACGGA GGAGGAGTTC CTGCGCGTGT GCCGGCTGAA 3361 GGAGAAGGAG CTGGAGGCCC TGCCGTGCCT GTACGCCACG GTGGAACCTG ACATGTGGGG 3421 CAGCGTAGAG GAGCTGCTCC GCGTTGTCAA GGACAAGATC GGCGAGGAGC AGCGCAAGAC 3481 CATCTGGGTG GACGAGGACC AGCTGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG 3541 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 3601 ACTTGAAATA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACTTGTGCAA 3661 ACTCCCCACC CAACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa 3721 gatt