Construct: ORF TRCN0000472387

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009993.1_s317c1
Derived from:
ccsbBroadEn_00882
DNA Barcode:
CGGGCCTTGCAACTATCGGTTGCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IRS1 (3667)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472387
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3667 IRS1 insulin receptor substrate 1 NM_005544.2 100% 100%
2 mouse 16367 Irs1 insulin receptor substrate 1 NM_010570.4 85% 89.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3792
ORF length:
3726
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gagccctccg gagagcgatg gcttctcgga cgtgcgcaag gtgggctacc
121tgcgcaaacc caagagcatg cacaaacgct tcttcgtact gcgcgcggcc agcgaggctg
181ggggcccggc gcgcctcgag tactacgaga acgagaagaa gtggcggcac aagtcgagcg
241cccccaaacg ctcgatcccc cttgagagct gcttcaacat caacaagcgg gctgactcca
301agaacaagca cctggtggct ctctacaccc gggacgagca ctttgccatc gcggcggaca
361gcgaggccga gcaagacagc tggtaccagg ctctcctaca gctgcacaac cgtgctaagg
421gccaccacga cggagctgcg gccctcgggg cgggaggtgg tgggggcagc tgcagcggca
481gctccggcct tggtgaggct ggggaggact tgagctacgg tgacgtgccc ccaggacccg
541cattcaaaga ggtctggcaa gtgatcctga agcccaaggg cctgggtcag acaaagaacc
601tgattggtat ctaccgcctt tgcctgacca gcaagaccat cagcttcgtg aagctgaact
661cggaggcagc ggccgtggtg ctgcagctga tgaacatcag gcgctgtggc cactcggaaa
721acttcttctt catcgaggtg ggccgttctg ccgtgacggg gcccggggag ttctggatgc
781aggtggatga ctctgtggtg gcccagaaca tgcacgagac catcctggag gccatgcggg
841ccatgagtga tgagttccgc cctcgcagca agagccagtc ctcgtccaac tgctctaacc
901ccatcagcgt ccccctgcgc cggcaccatc tcaacaatcc cccgcccagc caggtggggc
961tgacccgccg atcacgcact gagagcatca ccgccacctc cccggccagc atggtgggcg
1021ggaagccagg ctccttccgt gtccgcgcct ccagtgacgg cgaaggcacc atgtcccgcc
1081cagcctcggt ggacggcagc cctgtgagtc ccagcaccaa cagaacccac gcccaccggc
1141atcggggcag cgcccggctg caccccccgc tcaaccacag ccgctccatc cccatgccgg
1201cttcccgctg ctcgccttcg gccaccagcc cggtcagtct gtcgtccagt agcaccagtg
1261gccatggctc cacctcggat tgtctcttcc cacggcgatc tagtgcttcg gtgtctggtt
1321cccccagcga tggcggtttc atctcctcgg atgagtatgg ctccagtccc tgcgatttcc
1381ggagttcctt ccgcagtgtc actccggatt ccctgggcca caccccacca gcccgcggtg
1441aggaggagct aagcaactat atctgcatgg gtggcaaggg gccctccacc ctgaccgccc
1501ccaacggtca ctacattttg tctcggggtg gcaatggcca ccgctgcacc ccaggaacag
1561gcttgggcac gagtccagcc ttggctgggg atgaagcagc cagtgctgca gatctggata
1621atcggttccg aaagagaact cactcggcag gcacatcccc taccattacc caccagaaga
1681ccccgtccca gtcctcagtg gcttccattg aggagtacac agagatgatg cctgcctacc
1741caccaggagg tggcagtgga ggccgactgc cgggacacag gcactccgcc ttcgtgccca
1801cccgctccta cccagaggag ggtctggaaa tgcacccctt ggagcgtcgg ggggggcacc
1861accgcccaga cagctccacc ctccacacgg atgatggcta catgcccatg tccccagggg
1921tggccccagt gcccagtggc cgaaagggca gtggagacta tatgcccatg agccccaaga
1981gcgtatctgc cccacagcag atcatcaatc ccatcagacg ccatccccag agagtggacc
2041ccaatggcta catgatgatg tcccccagcg gtggctgctc tcctgacatt ggaggtggcc
2101ccagcagcag cagcagcagc agcaacgccg tcccttccgg gaccagctat ggaaagctgt
2161ggacaaacgg ggtagggggc caccactctc atgtcttgcc tcaccccaaa cccccagtgg
2221agagcagcgg tggtaagctc ttaccttgca caggtgacta catgaacatg tcaccagtgg
2281gggactccaa caccagcagc ccctccgact gctactacgg ccctgaggac ccccagcaca
2341agccagtcct ctcctactac tcattgccaa gatcctttaa gcacacccag cgccccgggg
2401agccggagga gggtgcccgg catcagcacc tccgcctttc cactagctct ggtcgccttc
2461tctatgctgc aacagcagat gattcttcct cttccaccag cagcgacagc ctgggtgggg
2521gatactgcgg ggctaggctg gagcccagcc ttccacatcc ccaccatcag gttctgcagc
2581cccatctgcc tcgaaaggtg gacacagctg ctcagaccaa tagccgcctg gcccggccca
2641cgaggctgtc cctgggggat cccaaggcca gcaccttacc tcgggcccga gagcagcagc
2701agcagcagca gcccttgctg caccctccag agcccaagag cccgggggaa tatgtcaata
2761ttgaatttgg gagtgatcag tctggctact tgtctggccc ggtggctttc cacagctcac
2821cttctgtcag gtgtccatcc cagctccagc cagctcccag agaggaagag actggcactg
2881aggagtacat gaagatggac ctggggccgg gccggagggc agcctggcag gagagcactg
2941gggtcgagat gggcagactg ggccctgcac ctcccggggc tgctagcatt tgcaggccta
3001cccgggcagt gcccagcagc cggggtgact acatgaccat gcagatgagt tgtccccgtc
3061agagctacgt ggacacctcg ccagctgccc ctgtaagcta tgctgacatg cgaacaggca
3121ttgctgcaga ggaggtgagc ctgcccaggg ccaccatggc tgctgcctcc tcatcctcag
3181cagcctctgc ttccccgact gggcctcaag gggcagcaga gctggctgcc cactcgtccc
3241tgctgggggg cccacaagga cctgggggca tgagcgcctt cacccgggtg aacctcagtc
3301ctaaccgcaa ccagagtgcc aaagtgatcc gtgcagaccc acaagggtgc cggcggaggc
3361atagctccga gactttctcc tcaacaccca gtgccacccg ggtgggcaac acagtgccct
3421ttggagcggg ggcagcagta gggggcggtg gcggtagcag cagcagcagc gaggatgtga
3481aacgccacag ctctgcttcc tttgagaatg tgtggctgag gcctggggag cttgggggag
3541cccccaagga gccagccaaa ctgtgtgggg ctgcTGGGGG TTTGGAGAAT GGTCTTAACT
3601ACATAGACCT GGATTTGGTC AAGGACTTCA AACAGTGCCC TCAGGAGTGC ACCCCTGAAC
3661CGCAGCCTCC CCCACCCCCA CCCCCTCATC AACCCCTGGG CAGCGGTGAG AGCAGCTCCA
3721CCCGCCGCTC AAGTGAGGAT TTAAGCGCCT ATGCCAGCAT CAGTTTCCAG AAGCAGCCAG
3781AGGACCGTCA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
3841ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
3901CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGG GCCTTGCAAC TATCGGTTGC
3961GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)