Construct: ORF TRCN0000472387
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009993.1_s317c1
- Derived from:
- ccsbBroadEn_00882
- DNA Barcode:
- CGGGCCTTGCAACTATCGGTTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IRS1 (3667)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472387
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3667 | IRS1 | insulin receptor substrate 1 | NM_005544.2 | 100% | 100% | |
| 2 | mouse | 16367 | Irs1 | insulin receptor substrate 1 | NM_010570.4 | 85% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3792
- ORF length:
- 3726
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gagccctccg gagagcgatg gcttctcgga cgtgcgcaag gtgggctacc 121 tgcgcaaacc caagagcatg cacaaacgct tcttcgtact gcgcgcggcc agcgaggctg 181 ggggcccggc gcgcctcgag tactacgaga acgagaagaa gtggcggcac aagtcgagcg 241 cccccaaacg ctcgatcccc cttgagagct gcttcaacat caacaagcgg gctgactcca 301 agaacaagca cctggtggct ctctacaccc gggacgagca ctttgccatc gcggcggaca 361 gcgaggccga gcaagacagc tggtaccagg ctctcctaca gctgcacaac cgtgctaagg 421 gccaccacga cggagctgcg gccctcgggg cgggaggtgg tgggggcagc tgcagcggca 481 gctccggcct tggtgaggct ggggaggact tgagctacgg tgacgtgccc ccaggacccg 541 cattcaaaga ggtctggcaa gtgatcctga agcccaaggg cctgggtcag acaaagaacc 601 tgattggtat ctaccgcctt tgcctgacca gcaagaccat cagcttcgtg aagctgaact 661 cggaggcagc ggccgtggtg ctgcagctga tgaacatcag gcgctgtggc cactcggaaa 721 acttcttctt catcgaggtg ggccgttctg ccgtgacggg gcccggggag ttctggatgc 781 aggtggatga ctctgtggtg gcccagaaca tgcacgagac catcctggag gccatgcggg 841 ccatgagtga tgagttccgc cctcgcagca agagccagtc ctcgtccaac tgctctaacc 901 ccatcagcgt ccccctgcgc cggcaccatc tcaacaatcc cccgcccagc caggtggggc 961 tgacccgccg atcacgcact gagagcatca ccgccacctc cccggccagc atggtgggcg 1021 ggaagccagg ctccttccgt gtccgcgcct ccagtgacgg cgaaggcacc atgtcccgcc 1081 cagcctcggt ggacggcagc cctgtgagtc ccagcaccaa cagaacccac gcccaccggc 1141 atcggggcag cgcccggctg caccccccgc tcaaccacag ccgctccatc cccatgccgg 1201 cttcccgctg ctcgccttcg gccaccagcc cggtcagtct gtcgtccagt agcaccagtg 1261 gccatggctc cacctcggat tgtctcttcc cacggcgatc tagtgcttcg gtgtctggtt 1321 cccccagcga tggcggtttc atctcctcgg atgagtatgg ctccagtccc tgcgatttcc 1381 ggagttcctt ccgcagtgtc actccggatt ccctgggcca caccccacca gcccgcggtg 1441 aggaggagct aagcaactat atctgcatgg gtggcaaggg gccctccacc ctgaccgccc 1501 ccaacggtca ctacattttg tctcggggtg gcaatggcca ccgctgcacc ccaggaacag 1561 gcttgggcac gagtccagcc ttggctgggg atgaagcagc cagtgctgca gatctggata 1621 atcggttccg aaagagaact cactcggcag gcacatcccc taccattacc caccagaaga 1681 ccccgtccca gtcctcagtg gcttccattg aggagtacac agagatgatg cctgcctacc 1741 caccaggagg tggcagtgga ggccgactgc cgggacacag gcactccgcc ttcgtgccca 1801 cccgctccta cccagaggag ggtctggaaa tgcacccctt ggagcgtcgg ggggggcacc 1861 accgcccaga cagctccacc ctccacacgg atgatggcta catgcccatg tccccagggg 1921 tggccccagt gcccagtggc cgaaagggca gtggagacta tatgcccatg agccccaaga 1981 gcgtatctgc cccacagcag atcatcaatc ccatcagacg ccatccccag agagtggacc 2041 ccaatggcta catgatgatg tcccccagcg gtggctgctc tcctgacatt ggaggtggcc 2101 ccagcagcag cagcagcagc agcaacgccg tcccttccgg gaccagctat ggaaagctgt 2161 ggacaaacgg ggtagggggc caccactctc atgtcttgcc tcaccccaaa cccccagtgg 2221 agagcagcgg tggtaagctc ttaccttgca caggtgacta catgaacatg tcaccagtgg 2281 gggactccaa caccagcagc ccctccgact gctactacgg ccctgaggac ccccagcaca 2341 agccagtcct ctcctactac tcattgccaa gatcctttaa gcacacccag cgccccgggg 2401 agccggagga gggtgcccgg catcagcacc tccgcctttc cactagctct ggtcgccttc 2461 tctatgctgc aacagcagat gattcttcct cttccaccag cagcgacagc ctgggtgggg 2521 gatactgcgg ggctaggctg gagcccagcc ttccacatcc ccaccatcag gttctgcagc 2581 cccatctgcc tcgaaaggtg gacacagctg ctcagaccaa tagccgcctg gcccggccca 2641 cgaggctgtc cctgggggat cccaaggcca gcaccttacc tcgggcccga gagcagcagc 2701 agcagcagca gcccttgctg caccctccag agcccaagag cccgggggaa tatgtcaata 2761 ttgaatttgg gagtgatcag tctggctact tgtctggccc ggtggctttc cacagctcac 2821 cttctgtcag gtgtccatcc cagctccagc cagctcccag agaggaagag actggcactg 2881 aggagtacat gaagatggac ctggggccgg gccggagggc agcctggcag gagagcactg 2941 gggtcgagat gggcagactg ggccctgcac ctcccggggc tgctagcatt tgcaggccta 3001 cccgggcagt gcccagcagc cggggtgact acatgaccat gcagatgagt tgtccccgtc 3061 agagctacgt ggacacctcg ccagctgccc ctgtaagcta tgctgacatg cgaacaggca 3121 ttgctgcaga ggaggtgagc ctgcccaggg ccaccatggc tgctgcctcc tcatcctcag 3181 cagcctctgc ttccccgact gggcctcaag gggcagcaga gctggctgcc cactcgtccc 3241 tgctgggggg cccacaagga cctgggggca tgagcgcctt cacccgggtg aacctcagtc 3301 ctaaccgcaa ccagagtgcc aaagtgatcc gtgcagaccc acaagggtgc cggcggaggc 3361 atagctccga gactttctcc tcaacaccca gtgccacccg ggtgggcaac acagtgccct 3421 ttggagcggg ggcagcagta gggggcggtg gcggtagcag cagcagcagc gaggatgtga 3481 aacgccacag ctctgcttcc tttgagaatg tgtggctgag gcctggggag cttgggggag 3541 cccccaagga gccagccaaa ctgtgtgggg ctgcTGGGGG TTTGGAGAAT GGTCTTAACT 3601 ACATAGACCT GGATTTGGTC AAGGACTTCA AACAGTGCCC TCAGGAGTGC ACCCCTGAAC 3661 CGCAGCCTCC CCCACCCCCA CCCCCTCATC AACCCCTGGG CAGCGGTGAG AGCAGCTCCA 3721 CCCGCCGCTC AAGTGAGGAT TTAAGCGCCT ATGCCAGCAT CAGTTTCCAG AAGCAGCCAG 3781 AGGACCGTCA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 3841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 3901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGG GCCTTGCAAC TATCGGTTGC 3961 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t