Construct: ORF TRCN0000472412
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012506.1_s317c1
- Derived from:
- ccsbBroadEn_06843
- DNA Barcode:
- CCCCCGGATATCTCCACGCACGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RARS1 (5917)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472412
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5917 | RARS1 | arginyl-tRNA synthetase 1 | NM_002887.4 | 99.9% | 100% | 1497G>A |
| 2 | mouse | 104458 | Rars | arginyl-tRNA synthetase | NM_025936.3 | 87% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2046
- ORF length:
- 1980
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgtactggtg tctgagtgct ccgcgcggct gctgcagcag gaagaagaga 121 ttaaatctct gactgctgaa attgaccggt tgaaaaactg tggctgttta ggagcttctc 181 caaatttgga gcagttacaa gaagaaaatt taaaattaaa gtatcgactg aatattcttc 241 gaaagagtct tcaggcagaa aggaacaaac caactaaaaa tatgattaac attattagcc 301 gcctacaaga ggtctttggt catgcaatta aggctgcata tccagatttg gaaaatcctc 361 ctctgctagt gacaccaagt cagcaggcca agtttgggga ctatcagtgt aatagtgcta 421 tgggtatttc tcagatgctc aaaaccaagg aacagaaagt taatccaaga gaaattgctg 481 aaaacattac caaacacctc ccagacaatg aatgtattga aaaagttgaa attgctggtc 541 ctggttttat taatgtccac ttaagaaagg attttgtatc agaacaattg accagtcttc 601 tagtgaatgg agttcaacta cctgctctgg gagagaataa aaaggttata gttgactttt 661 cctcccctaa tatagctaaa gagatgcatg taggccacct gaggtcaact atcataggag 721 agagtataag ccgcctcttt gaatttgcag ggtatgacgt gctcaggtta aatcatgtag 781 gagactgggg gacccagttt ggcatgctca tcgctcacct gcaagacaaa tttccagatt 841 atctaacagt ttcacctcct attggggatc ttcaggtctt ttataaggaa tctaagaaga 901 ggtttgatac tgaggaggaa tttaagaagc gagcatatca gtgtgtagtt ctgctccagg 961 gtaaaaaccc agatattaca aaagcttgga agcttatctg tgatgtctcc cgccaagagt 1021 taaataaaat ctatgatgca ttggacgtct ctttaataga gagaggggaa tccttctatc 1081 aagataggat gaatgatatt gtaaaggaat ttgaagatag aggatttgtg caggtggatg 1141 atggcagaaa gattgtattt gtcccagggt gttccatacc attaaccata gtaaaatcag 1201 atggaggtta tacctatgat acatctgacc tggctgctat taaacaaaga ctatttgagg 1261 aaaaagcaga tatgattatc tatgttgtgg acaatggaca atctgtgcac ttccagacaa 1321 tatttgctgc tgctcaaatg attggttggt atgaccctaa agtaactcga gtcttccatg 1381 ctggatttgg tgtggtgcta ggggaagaca agaaaaagtt taaaacacgt tcgggtgaaa 1441 cagtgcgcct catggatctt ctgggagaag gactaaaacg atccatggac aagttgaagg 1501 aaaaagaaag agacaaggtc ttaactgcag aggaattgaa tgctgctcag acatccgttg 1561 catatggctg catcaaatat gctgaccttt cccataaccg gttgaatgac tacatcttct 1621 cctttgacaa aaTGCTAGAT GACAGAGGAA ATACAGCTGC TTACTTGTTG TATGCCTTCA 1681 CTAGAATCAG GTCTATTGCA CGTCTGGCCA ATATTGATGA AGAAATGCTC CAAAAAGCTG 1741 CTCGAGAAAC CAAGATTCTT TTGGATCATG AGAAGGAATG GAAACTAGGC CGGTGCATTT 1801 TACGGTTCCC TGAGATTCTG CAAAAGATTT TAGATGACTT ATTTCTCCAC ACTCTCTGTG 1861 ATTATATATA TGAGCTGGCA ACTGCTTTCA CAGAGTTCTA TGATAGCTGC TACTGTGTGG 1921 AGAAAGATAG ACAGACTGGA AAAATATTGA AGGTGAACAT GTGGCGTATG CTGCTATGTG 1981 AAGCAGTAGC TGCTGTCATG GCCAAGGGGT TTGATATCCT GGGAATAAAA CCTGTCCAAA 2041 GGATGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2101 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2161 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCCCGGAT ATCTCCACGC ACGCGACGCG 2221 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt