Construct: ORF TRCN0000472420
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009391.1_s317c1
- Derived from:
- ccsbBroadEn_12739
- DNA Barcode:
- GCCCCCCCCAGCTGCCACAGACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTC25 (83538)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472420
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83538 | TTC25 | tetratricopeptide repeat do... | NM_031421.5 | 98.8% | 98.5% | 651C>T;1980_1981insA;1995_2016del |
| 2 | human | 83538 | TTC25 | tetratricopeptide repeat do... | NM_001350319.2 | 72.1% | 67.6% | 651C>T;1342_1343ins101;1440_1441ins454 |
| 3 | human | 83538 | TTC25 | tetratricopeptide repeat do... | NR_146622.2 | 60% | (many diffs) | |
| 4 | human | 83538 | TTC25 | tetratricopeptide repeat do... | NR_110662.3 | 49.6% | (many diffs) | |
| 5 | human | 83538 | TTC25 | tetratricopeptide repeat do... | NR_146621.2 | 46.3% | (many diffs) | |
| 6 | mouse | 74407 | Ttc25 | tetratricopeptide repeat do... | XM_011249287.2 | 66.2% | 67.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2061
- ORF length:
- 1995
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggaccccgaa ggcgagacct tgcgaagcac ctttccctct tatatggccg 121 aaggcgagcg gctctacctg tgcggggaat tttctaaagc cgcgcagagc ttcagcaacg 181 ctctttacct tcaggatgga gacaagaact gcctggttgc tcgctcaaag tgcttcctga 241 agatgggaga cttggagaga tccctgaagg atgctgaggc ttcgctccag agtgacccag 301 ctttctgtaa ggggattttg caaaaggctg agacactgta caccatggga gactttgagt 361 ttgccttggt attctatcat cgaggctaca agctgaggcc tgatcgggaa ttcagagttg 421 gcattcagaa agcccaggaa gccatcaaca actcagtggg aagtccttct tccattaagc 481 tggagaacaa aggggacctc tccttcttaa gcaagcaggc tgagaatata aaagcccagc 541 agaagcctca gcccatgaaa cacctcttac accccaccaa gggagagccc aagtggaagg 601 cctcgctcaa gagtgagaag actgtccgcc agcttctggg ggagctctac gtggacaaag 661 agtatttgga gaagctccta ttggatgaag acctgatcaa aggcaccatg aagggtggcc 721 tgactgtgga ggacctcatc atgacgggca tcaactacct ggatactcac agcaacttct 781 ggaggcagca gaagccgatc tacgccaggg agcgggaccg gaagctgatg caagagaaat 841 ggctgcggga ccacaaacgc cgtccctcac agacagccca ttacatcctc aagagcctgg 901 aggacattga tatgttgctc acaagtggca gtgctgaagg gagtcttcag aaagctgaga 961 aagtgctgaa gaaggtactg gaatggaaca aggaagaggt acccaacaag gatgaactgg 1021 ttggaaactt gtatagctgc atagggaatg cccagattga gctggggcag atggaggcag 1081 ccctgcagag ccacagaaag gacctggaga tcgccaagga atatgacctt cctgatgcaa 1141 aatcgagagc ccttgacaac attggcagag tttttgccag agttgggaaa ttccagcaag 1201 ccattgacac gtgggaagaa aagatccctc tggcaaaaac caccctggag aagacctggc 1261 tgttccacga gatcggccgc tgctacttgg agctggacca ggcctggcag gcccagaatt 1321 atggcgagaa gtcccagcag tgtgccgagg aggaagggga cattgagtgg caactgaatg 1381 ccagtgttct ggtggcccag gcacaagtga agctgagaga cttcgagtca gccgtgaaca 1441 attttgagaa ggccctggag agagcaaagc ttgtgcataa caacgaggcg cagcaggcca 1501 tcatcagtgc cttggacgat gccaacaagg gtatcatcag agaactgagg aaaaccaact 1561 acgtggagaa tctcaaagaa aaaagcgagg gagaagcttc actgtatgaa gatagaataa 1621 taacaagaga gaaggaCATG AGGAGAGTGA GAGATGAGCC CGAGAAGGTG GTGAAGCAGT 1681 GGGACCATAG TGAGGATGAG AAAGAGACAG ATGAGGACGA TGAGGCTTTT GGGGAAGCTC 1741 TGCAGAGCCC AGCAAGCGGA AAGCAGAGTG TGGAAGCAGG AAAAGCCAGA AGCGATTTGG 1801 GAGCAGTTGC CAAGGGCCTG TCAGGAGAAT TAGGCACAAG ATCAGGAGAA ACAGGCAGGA 1861 AGCTACTAGA AGCTGGCAGA AGAGAGTCAA GAGAAATTTA TAGGAGGCCT TCGGGAGAAT 1921 TAGAGCAAAG ACTCTCAGGA GAATTCAGCA GACAGGAACC AGAAGAACTA AAGAAACTTT 1981 CAGAAGTGGG CAGAAGAGAG CCAGAAGAAC TGGGAAAAAC ACAATTTGGA GAAATAGGAG 2041 AAACGAAAAA AAACAGGAAA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2101 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2161 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCCC CCCCCAGCTG 2221 CCACAGACGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt