Construct: ORF TRCN0000472422
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012129.1_s317c1
- Derived from:
- ccsbBroadEn_14065
- DNA Barcode:
- AGGCCGGAGTGTTCCCAGATCCTT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC23IP (11196)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472422
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11196 | SEC23IP | SEC23 interacting protein | NM_007190.4 | 99.9% | 99.6% | 2986delG |
| 2 | human | 11196 | SEC23IP | SEC23 interacting protein | XM_005269469.3 | 99.8% | 99.5% | 2756_2757insAGC;2983delG |
| 3 | human | 11196 | SEC23IP | SEC23 interacting protein | XM_005269470.2 | 95% | 94% | (many diffs) |
| 4 | human | 11196 | SEC23IP | SEC23 interacting protein | XM_017015610.1 | 94.9% | 93.9% | (many diffs) |
| 5 | human | 11196 | SEC23IP | SEC23 interacting protein | XR_246061.2 | 68.9% | 1_87del;3073delG;3088_4352del | |
| 6 | human | 11196 | SEC23IP | SEC23 interacting protein | XR_002956945.1 | 39.5% | (many diffs) | |
| 7 | human | 11196 | SEC23IP | SEC23 interacting protein | NR_037771.2 | 34.4% | (many diffs) | |
| 8 | mouse | 207352 | Sec23ip | Sec23 interacting protein | NM_001029982.2 | 84.8% | 86% | (many diffs) |
| 9 | mouse | 207352 | Sec23ip | Sec23 interacting protein | XM_006507519.3 | 84.7% | 85.9% | (many diffs) |
| 10 | mouse | 207352 | Sec23ip | Sec23 interacting protein | XM_006507521.2 | 65.8% | 67.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3066
- ORF length:
- 3000
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgagagaaaa cctaacggtg gcagcggcgg cgcctccact tcctcatcgg 121 gcactaactt acttttctcc tcctcggcca cggagttcag cttcaatgtg cccttcatcc 181 cagtcaccca ggcctccgct tctccggcct ccctgctctt accgggagag gattccacag 241 atgttggtga ggaggacagc ttccttggtc agacttctat tcacacatct gccccacaga 301 catttagtta cttctctcag gtatcaagca gcagtgatcc ttttgggaat attggacagt 361 caccattaac aactgcagca acctcagttg gacaatcagg attccccaag cccctgactg 421 ctctcccttt tacaactgga tcccaagatg tctcgaatgc attttcacca tccatttcga 481 aggctcaacc tggtgctcca ccttcctcac tgatgggaat aaattcttat ctgccttctc 541 agccaagtag tctccctcct tcatattttg ggaaccaacc ccaaggaatt ccccaaccag 601 gatacaatcc atatcgccat acccctggca gcagcagggc taatccttac attgcaccac 661 cccagctgca gcagtgccaa acaccaggcc ctcctgctca tcctccacct tctggacccc 721 ctgttcagat gtaccagatg cctccaggat ctttgccacc ggttccttct tcagtgcagt 781 caccggcaca gcagcaggta cctgccagac ctggggctcc ctctgttcaa gtgccatctc 841 cttttctact tcaaaaccaa tatgagcctg ttcagcccca ctggttttac tgcaaggagg 901 tagaatacaa acaactgtgg atgcctttta gtgtgttcga ctctttgaat cttgaagaaa 961 tctataattc agttcagcca gatccggaga gcgtggttct tggcacggat ggagggcgct 1021 acgatgttta cctctatgac cgaataagga aggctgccta ctgggaagag gagccagccg 1081 aagtgagacg ctgtacttgg ttttacaagg gggacacaga tagtcgattt attccctata 1141 ctgaggagtt cagtgaaaaa ctagaggctg aatataaaaa agctgtaacc actaatcagt 1201 ggcaccgaag attagagttt ccaagtggag agacaattgt tatgcacaat ccaaaggtta 1261 ttgttcagtt ccagccctcc tcagtgccag atgaatgggg caccacgcaa gatggacaga 1321 caaggcccag ggttgtaaag cgtggaattg atgataacct tgatgaaatt cccgacgggg 1381 agatgcctca agttgaccat ttggtgtttg tggtgcatgg cattggacct gtgtgtgact 1441 tacgctttag gagcattatt gagtgtgtgg atgattttag ggtggtttct ctcaaattgc 1501 tgcggacaca tttcaagaaa tctttagatg acgggaaagt aagcagagtg gagttccttc 1561 cagttcattg gcatagttct ttgggtgggg acgccacagg tgtggacagg aatattaaga 1621 aaatcacttt gccaagtatt ggtcgatttc gtcactttac caatgaaact ttgctagata 1681 ttttatttta taacagcccc acctactgtc agacaattgt ggaaaaagta ggaatggaga 1741 taaaccatct gcatgcactc tttatgagtc ggaacccaga cttcaaagga ggtgtctctg 1801 ttgctggtca cagtttaggt tctttaatat tgtttgacat cctgtctaat caaaaagatt 1861 tgaatttatc aaagtgccct ggacctcttg ctgttgctaa tggagttgtg aagcagctac 1921 attttcagga aaagcagatg cctgaagagc caaagctgac tttggatgag tcgtatgacc 1981 ttgttgttga aaataaagaa gtcctaactt tgcaagaaac tctggaagca cttagcctct 2041 ctgaatattt tagcactttt gaaaaggaaa agattgatat ggagtccctg cttatgtgta 2101 cagttgatga cctgaaggaa atggggatac cccttggacc cagaaagaag atagctaact 2161 ttgtagaaca taaagcagcc aaactgaaaa aagcagcgtc agaaaagaag gcagtggcgg 2221 ccacttctac aaaaggacaa gagcaaagtg cccagaagac taaagacatg gcttccctcc 2281 cctcagaatc caatgagcca aagaggaaac ttccagttgg tgcttgcgtg tcttctgtgt 2341 gtgtgaatta tgaatctttt gaagttggcg ccggacaggt ttctgttgct tacaactcat 2401 tagattttga accagagata ttctttgcct tggggtctcc aattgctatg tttctcacta 2461 ttcgaggagt tgataggata gatgagaatt acagccttcc tacctgtaaa gggttcttca 2521 atatttatca tccgcttgat ccagtggcat atagattaga acctatgatt gttccagatt 2581 tggacctaaa agctgttctc attccacatc acaaaggcag aaaaagactt catttagaat 2641 tgaaagagag tctctctcgt atgggatctg atttgaagca gggTTTTATT AGCTCTCTCA 2701 AAAGTGCTTG GCAGACATTA AATGAGTTTG CCCGTGCTCA TACGTCTTCA ACCCAGTTGC 2761 AAGAAGAATT GGAGAAGGTG GCCAATCAGA TCAAAGAAGA AGAAGAAAAG CAAGTAGTTG 2821 AAGCAGAAAA GGTTGTTGAA AGTCCAGATT TTTCCAAGGA TGAGGACTAC TTAGGAAAGG 2881 TTGGAATGTT AAATGGAGGC CGCCGAATTG ACTACGTTCT CCAAGAAAAA CCAATAGAGA 2941 GTTTTAATGA ATACCTTTTC GCTCTTCAGA GTCACTTATG CTATTGGGAA TCTGAAGATA 3001 CTGCTCTGTT ACTACTTAAA GAAATTTATC GAACAATGAA CATTAGTCCA AACAGCCCCA 3061 GCATTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 3121 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 3181 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGGCCGGAGT GTTCCCAGAT CCTTACGCGT 3241 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt