Construct: ORF TRCN0000472426

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006046.1_s317c1
Derived from:
ccsbBroadEn_03549
DNA Barcode:
ATCTACAGGTGCTTCTTTGCGACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DCUN1D2 (55208)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472426
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55208 DCUN1D2 defective in cullin neddyla... NM_001014283.2 100% 100%
2 human 55208 DCUN1D2 defective in cullin neddyla... XM_017020644.1 94.2% 94.2% 0_1ins45
3 human 55208 DCUN1D2 defective in cullin neddyla... XM_017020646.2 94.2% 94.2% 0_1ins45
4 human 55208 DCUN1D2 defective in cullin neddyla... XM_017020647.1 94.2% 94.2% 0_1ins45
5 human 55208 DCUN1D2 defective in cullin neddyla... XM_017020648.1 94.2% 94.2% 0_1ins45
6 human 55208 DCUN1D2 defective in cullin neddyla... XM_017020649.1 81.8% 73.7% (many diffs)
7 human 55208 DCUN1D2 defective in cullin neddyla... XM_017020643.1 76.5% 76.5% 1_236del;237_238insTG
8 human 55208 DCUN1D2 defective in cullin neddyla... XR_245842.3 24.4% 1_40del;560_711del;970_3182del
9 mouse 102323 Dcun1d2 DCN1, defective in cullin n... NM_001024504.2 87% 91.1% (many diffs)
10 mouse 102323 Dcun1d2 DCN1, defective in cullin n... XM_006508684.2 81.7% 85.7% (many diffs)
11 mouse 102323 Dcun1d2 DCN1, defective in cullin n... NM_001042651.1 69.3% 70.6% (many diffs)
12 mouse 102323 Dcun1d2 DCN1, defective in cullin n... NM_001042650.1 66.4% 68.7% (many diffs)
13 mouse 102323 Dcun1d2 DCN1, defective in cullin n... NM_001042649.1 65.8% 58.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
843
ORF length:
777
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca taagcttaaa tcgtctcaga aggacaaggt ccgccagttt atggcgtgca
121ctcaggctgg cgagagaact gctatctact gcttaacgca gaatgagtgg agactagacg
181aggccacgga cagcttcttc caaaacccag actcgctcca cagggagtcc atgcggaacg
241ctgtggacaa gaagaagctg gagcggctgt acggcaggta caaagatcca caagatgaaa
301acaaaattgg agtcgatggg attcaacagt tttgtgatga tctgagcctg gatcctgcca
361gtatcagtgt attggtcata gcgtggaagt tcagggcagc aactcagtgt gaatttagca
421gaaaggaatT TCTAGATGGC ATGACAGAAC TTGGGTGTGA CAGCATGGAG AAGCTAAAGG
481CTCTTCTGCC AAGACTGGAG CAGGAGCTGA AGGACACAGC CAAGTTTAAA GATTTTTATC
541AGTTTACCTT CACCTTCGCT AAGAACCCAG GGCAGAAAGG TTTAGACTTA GAAATGGCTG
601TTGCGTATTG GAAATTAGTG TTATCTGGAA GGTTTAAATT TTTAGATCTC TGGAACACAT
661TCTTAATGGA ACATCACAAA AGATCAATTC CAAGGGACAC CTGGAACCTC CTGCTGGACT
721TTGGAAACAT GATTGCGGAT GATATGTCTA ACTACGATGA AGAAGGAGCT TGGCCCGTTC
781TTATAGATGA TTTTGTAGAA TATGCACGGC CAGTAGTCAC AGGTGGAAAA CGCAGCCTTT
841TCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
901TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
961GGCTTTATAT ATCTTGTGGA AAGGACGAAT CTACAGGTGC TTCTTTGCGA CAACGCGTTA
1021AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)