Construct: ORF TRCN0000472442
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004224.1_s317c1
- Derived from:
- ccsbBroadEn_03920
- DNA Barcode:
- CGCAATTCACGTGATTGATGTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAMSN1 (64092)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472442
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64092 | SAMSN1 | SAM domain, SH3 domain and ... | NM_022136.5 | 100% | 100% | |
| 2 | human | 64092 | SAMSN1 | SAM domain, SH3 domain and ... | XM_011529684.2 | 95% | 94.9% | 0_1ins54;2T>A |
| 3 | human | 64092 | SAMSN1 | SAM domain, SH3 domain and ... | XM_011529685.1 | 86.5% | 86.5% | 127_128ins150 |
| 4 | human | 64092 | SAMSN1 | SAM domain, SH3 domain and ... | XM_017028427.1 | 83.1% | 82.3% | (many diffs) |
| 5 | human | 64092 | SAMSN1 | SAM domain, SH3 domain and ... | XM_011529686.1 | 82.7% | 81.8% | 919_920ins45;922C>G;927_928ins147 |
| 6 | human | 64092 | SAMSN1 | SAM domain, SH3 domain and ... | NM_001256370.1 | 82.3% | 80.2% | (many diffs) |
| 7 | human | 64092 | SAMSN1 | SAM domain, SH3 domain and ... | NM_001286523.1 | 81.5% | 81.5% | 0_1ins207 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1185
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct caagagaaag ccatccaatg tttcagagaa ggagaaacat caaaaaccaa 121 agcgaagcag cagttttggg aatttcgatc gttttcggaa taattcttta tcaaaaccag 181 atgattcaac tgaggcacat gaaggagatc ccacaaatgg aagtggagaa caaagtaaaa 241 cttcaaataa tggaggcggt ttgggtaaaa aaatgagagc tatttcatgg acaatgaaga 301 aaaaagtggg taaaaagtac atcaaagccc tttctgagga aaaggatgag gaagatggag 361 agaatgccca cccatataga aacagtgacc ctgtgattgg gacccacaca gagaaggtgt 421 ccctcaaagc cagtgactcc atggatagtc tctacagtgg acagagctca tcaagtggca 481 taacaagctg ttcagatggt acaagtaacc gggacagctt tcgactggat gacgatggcc 541 cctattcagg accattctgt ggccgtgcca gagtgcatac ggatttcacg ccaagtccct 601 atgacactga ctccctcaaa atcaagaaag gagacatcat agacattatt tgcaaaacac 661 caatggggat gtggacagga atgttgaaca ataaagtgGG AAACTTCAAA TTCATTTATG 721 TGGATGTCAT CTCAGAAGAG GAAGCAGCCC CCAAGAAAAT AAAGGCAAAC CGAAGGAGTA 781 ACAGCAAAAA ATCCAAGACT CTGCAGGAGT TCCTAGAGAG GATTCATCTG CAGGAATACA 841 CCTCAACACT TTTGCTCAAT GGTTATGAGA CTCTAGAAGA TTTAAAAGAT ATAAAAGAGA 901 GTCACCTCAT TGAATTAAAT ATTGAAAACC CAGATGACAG AAGAAGGTTA CTATCAGCTG 961 CTGAAAACTT CCTTGAAGAA GAAATTATTC AAGAGCAAGA AAATGAACCT GAGCCCCTAT 1021 CCTTGAGCTC AGACATCTCC TTAAATAAGT CACAGTTAGA TGACTGCCCA AGGGACTCTG 1081 GTTGCTATAT CTCATCAGGA AATTCAGATA ATGGCAAAGA GGATCTGGAG TCTGAAAATC 1141 TGTCTGACAT GGTACATAAG ATTATTATCA CAGAGCCAAG TGACTGCCCA ACTTTCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1321 GAAAGGACGA CGCAATTCAC GTGATTGATG TCCCACGCGT TAAGTCgaca atcaacctct 1381 ggattacaaa atttgtgaaa gatt