Construct: ORF TRCN0000472446
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015698.1_s317c1
- Derived from:
- ccsbBroadEn_14230
- DNA Barcode:
- AGAGCAACGATACTTGAGCCGTAC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLTRN (57393)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472446
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57393 | CLTRN | collectrin, amino acid tran... | NM_020665.6 | 99.3% | 99.5% | 663_666delTCTC |
| 2 | human | 57393 | CLTRN | collectrin, amino acid tran... | XM_017029680.1 | 75.9% | 76.1% | 0_1ins156;507_510delTCTC |
| 3 | human | 57393 | CLTRN | collectrin, amino acid tran... | XM_024452411.1 | 75.9% | 76.1% | 0_1ins156;507_510delTCTC |
| 4 | human | 57393 | CLTRN | collectrin, amino acid tran... | XM_017029681.1 | 53% | 53.1% | 203_204ins309;354_357delTCTC |
| 5 | mouse | 57394 | Tmem27 | transmembrane protein 27 | NM_020626.2 | 86% | 84.2% | (many diffs) |
| 6 | mouse | 57394 | Tmem27 | transmembrane protein 27 | NM_001313719.1 | 41.1% | 40.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 729
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gtggctgctc ttttttctgg tgactgccat tcatgctgaa ctctgtcaac 121 caggtgcaga aaatgctttt aaagtgagac ttagtatcag aacagctctg ggagataaag 181 catatgcctg ggataccaat gaagaatacc tcttcaaagc gatgGTAGCT TTCTCCATGA 241 GAAAAGTTCC CAACAGAGAA GCAACAGAAA TTTCCCATGT CCTACTTTGC AATGTAACCC 301 AGAGGGTATC ATTCTGGTTT GTGGTTACAG ACCCTTCAAA AAATCACACC CTTCCTGCTG 361 TTGAGGTGCA ATCAGCCATA AGAATGAACA AGAACCGGAT CAACAATGCC TTCTTTCTAA 421 ATGACCAAAC TCTGGAATTT TTAAAAATCC CTTCCACACT TGCACCACCC ATGGACCCAT 481 CTGTGCCCAT CTGGATTATT ATATTTGGTG TGATATTTTG CATCATCATA GTTGCAATTG 541 CACTACTGAT TTTATCAGGG ATCTGGCAAC GTAGAAGAAA GAACAAAGAA CCATCTGAAG 601 TGGATGACGC TGAAGATAAG TGTGAAAACA TGATCACAAT TGAAAATGGC ATCCCCTCTG 661 ATCCCCTGGA CATGAAGGGA GGGCATATTA ATGATGCCTT CATGACAGAG GATGAGAGGC 721 TCACCCCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 781 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 841 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGAGCAA CGATACTTGA GCCGTACACG 901 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt