Construct: ORF TRCN0000472448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002298.1_s317c1
- Derived from:
- ccsbBroadEn_01443
- DNA Barcode:
- ATCTGACCCAGTCCAAGTCTTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL12 (6182)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472448
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6182 | MRPL12 | mitochondrial ribosomal pro... | NM_002949.4 | 100% | 100% | |
| 2 | mouse | 56282 | Mrpl12 | mitochondrial ribosomal pro... | NM_027204.2 | 84% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 660
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gccggcggcc gctcgccccc tgtgggggcc ttgccttggg cttcgggccg 121 ctgcgttccg ccttgccagg cgacaggTGC CATGTGTCTG TGCCGTGCGA CATATGAGGA 181 GCAGCGGCCA TCAGAGGTGT GAGGCCCTCG CTGGTGCACC CCTGGATAAC GCCCCCAAGG 241 AGTACCCCCC CAAGATACAG CAGCTGGTCC AGGACATCGC CAGCCTCACT CTCTTGGAAA 301 TCTCAGACCT CAACGAGCTC CTGAAGAAAA CGTTGAAGAT CCAGGATGTC GGGCTTGTGC 361 CGATGGGTGG TGTGATGTCT GGGGCTGTCC CTGCTGCAGC AGCCCAGGAG GCGGTGGAAG 421 AAGATATCCC CATAGCGAAA GAACGGACAC ATTTCACCGT CCGCCTGACC GAGGCGAAGC 481 CCGTGGACAA AGTGAAGCTG ATCAAGGAAA TCAAGAACTA CATCCAAGGC ATCAACCTCG 541 TCCAGGCAAA GAAGCTGGTG GAGTCCCTGC CCCAGGAAAT CAAAGCCAAT GTCGCCAAAG 601 CTGAGGCGGA GAAGATCAAG GCGGCCCTGG AGGCGGTGGG CGGCACCGTG GTTCTGGAGT 661 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 721 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 781 TTTATATATC TTGTGGAAAG GACGAATCTG ACCCAGTCCA AGTCTTGCGA CGCGTTAAGT 841 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt