Construct: ORF TRCN0000472468
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017022.1_s317c1
- Derived from:
- ccsbBroadEn_06091
- DNA Barcode:
- AAGTGACTTATGCTACTACCCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX1 (1653)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472468
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1653 | DDX1 | DEAD-box helicase 1 | NM_004939.3 | 99.9% | 100% | 912C>T |
| 2 | mouse | 104721 | Ddx1 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_134040.1 | 90.1% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2286
- ORF length:
- 2220
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccttctcc gagatgggtg taatgcctga gattgcacaa gctgtggaag 121 agatggattg gctcctccca actgatatcc aggctgaatc tatcccattg atcttaggag 181 gaggtgatgt acttatggct gcagaaacag gaagtggcaa aactggtgct tttagtattc 241 cagttatcca gatagtttat gaaactctga aagaccaaca ggaaggcaaa aaaggaaaaa 301 caacaattaa aactggtgct tcagtgctga acaaatggca gatgaaccca tatgacagag 361 gatctgcttt tgcaattggg tcagatggtc tttgttgtca aagcagagaa gtaaaggaat 421 ggcatgggtg tagagctact aaaggattaa tgaaagggaa acactactat gaagtatcct 481 gtcatgacca agggttatgc agggtcgggt ggtctaccat gcaggcctct ttggacctag 541 gtactgacaa gtttggattt ggctttggtg gaacaggaaa gaaatcccat aacaaacaat 601 ttgataatta tggagaggaa ttcactatgc atgataccat tggatgttac ctggatatag 661 ataagggaca tgtcaagttc tccaaaaatg gaaaagatct tggtctggca tttgaaatac 721 caccacatat gaaaaaccaa gccctctttc ctgcctgtgt tttgaagaat gctgaactga 781 aatttaactt cggtgaagag gaatttaagt ttccaccaaa agatggcttt gttgctcttt 841 ccaaggcacc ggatggttac attgtcaaat cacagcactc aggtaatgca caggtgacac 901 aaacaaagtt tctccccaat gctccgaaag ctctcattgt tgaaccttcc cgggagttag 961 ctgaacaaac tttgaataac atcaagcagt ttaagaaata cattgataat cctaaattaa 1021 gggagcttct gataattgga ggtgttgcag cccgggatca gctctctgtt ttggaaaatg 1081 gagtagatat agttgtaggt actccgggaa gactagatga cttggtgtca actggaaagc 1141 tgaacttatc tcaagttaga ttcctggtcc tggatgaagc tgatgggctt ctttctcaag 1201 gttattctga ttttataaat aggatgcaca atcagattcc tcaggttacc tctgatggaa 1261 aaagacttca ggtgattgtt tgctctgcca ctttgcattc tttcgatgta aagaaactgt 1321 ccgagaagat aatgcatttt cctacatggg ttgacttaaa aggagaagac tctgttccag 1381 atactgtaca ccatgttgtt gtcccagtaa atcccaaaac tgacagactc tgggaaaggc 1441 ttggaaagag ccacattaga actgatgatg tacatgcaaa agataacaca agacctggtg 1501 ctaatagtcc agagatgtgg tctgaagcta ttaaaatcct gaaaggggag tatgctgtcc 1561 gggcaatcaa ggaacataag atggatcaag caattatctt ctgtagaacc aaaattgact 1621 gtgataactt ggagcagtac tttatacaac aaggaggagg acctgataaa aaaggacacc 1681 agttctcatg tgtttgtctt catggtgaca gaaagcctca tgagagaaag caaaacttgg 1741 aaagatttaa gaaaggagat gtaagattct tgatttgcac agatgtagct gctagaggaa 1801 ttgataTCCA CGGTGTTCCT TATGTTATAA ATGTCACTCT GCCCGATGAA AAGCAAAACT 1861 ACGTACATCG AATTGGCAGA GTAGGAAGAG CTGAAAGGAT GGGTCTGGCA ATTTCCCTGG 1921 TGGCAACAGA AAAAGAAAAG GTTTGGTACC ATGTATGTAG CAGCCGTGGA AAAGGGTGTT 1981 ATAACACAAG ACTCAAGGAA GATGGAGGCT GTACCATATG GTACAACGAG ATGCAGTTAC 2041 TATCTGAGAT AGAAGAACAC CTGAACTGTA CCATTTCTCA GGTTGAGCCG GATATAAAGG 2101 TACCAGTGGA TGAATTTGAT GGGAAAGTTA CCTACGGTCA GAAAAGGGCT GCTGGTGGTG 2161 GAAGCTATAA AGGCCATGTG GATATTTTGG CACCTACTGT TCAAGAGTTG GCTGCCCTTG 2221 AAAAGGAGGC GCAGACATCT TTCCTGCATC TTGGCTACCT TCCTAACCAG CTGTTCAGAA 2281 CCTTCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2341 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2401 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAAGTGACTT ATGCTACTAC CCCAAACGCG 2461 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt